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- W1569679004 abstract "Gemcitabine은 다양한 고형암 치료에 사용되는 항암제이다. 혈장내에서 cytidine deaminase에 의해 빠르게 대사되며, 친수성 성질로 인해 역상 액체크로마토그라피(reversed-phase liquid chromatography)를 이용한 농도 분석이 어렵다. 본 연구에서는 역상칼럼(reversed-phase column)을 이용한 초고속 액체크로마토그라피(ultra-performance liquid chromatography, UPLC) 방법에 의해 빠르고 정확한 속력(velocity)과 최고효능(peak efficiency)를 갖춘 분석법을 개발하고자 하였다. Gemcitabine과 2'-deoxycytidine의 머무름 시간(retension time)은 293 nm에서 각각 3.2분과 2.1분이었다. 검량선의 직선성 검정은 <TEX>$0.1{sim}20{mu}g/ml$</TEX>의 농도범위에서 높은 직선성을 나타내었다(<TEX>$r^2$</TEX>>0.999). 일내(intra-day) 변이게수(coefficients of variation)와 일간(inter-day) 변이게수는 모두 10% 이하였다. 정확성(accuracy) 검정을 위한 일내 및 일간 평균농도 측정치가 97.3~113.5% 범위로 나타났다. 이러한 결과를 토대로, gemcitabine 농도를 측정하기 위한 새로운 분석법으로 빠르고 정확한 역상 UPLC 방법을 제안하고자 한다. Gemcitabine is an anticancer drug used to treat a variety of solid tumors. The drug is rapidly inactivated by cytidine deaminase in plasma and its hydrophilicity restricts the extent of quantification that is possible using reversed-phase liquid chromatography. In this paper, we report a rapid and precise method to analyze velocity and peak efficiency using ultra-performance liquid chromatography (UPLC) with a reversed-phase column. The retention periods of gemcitabine and 2'-deoxycytidine at 283 nm were 3.2 and 2.1 min, respectively. The assay provided highly linear results in the range of <TEX>$0.1{sim}20{mu}g/ml$</TEX> (<TEX>$r^2$</TEX> > 0.999). The coefficients of variation of the intra-day and inter-day assays were less than 10.0%. We observed that the estimated average concentrations of the intra-day and inter-day assays ranged from 97.3 to 113.5% to verify the accuracy. These results suggest that this new reversed-phase UPLC method is a rapid and reliable way of determining gemcitabine levels." @default.
- W1569679004 created "2016-06-24" @default.
- W1569679004 date "2009-12-30" @default.
- W1569679004 modified "2023-10-14" @default.
- W1569679004 title "The Rapid Determination of Gemcitabine by Reversed-phase Ultra-Performance Liquid Chromatography" @default.
- W1569679004 doi "https://doi.org/10.5352/jls.2009.19.12.1698" @default.
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