FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569754812> ?p ?o ?g. }

• W1569754812 endingPage "3098" @default.
• W1569754812 startingPage "3092" @default.
• W1569754812 abstract "This paper presents the first complete purification of the branched chain aminotransferase (EC 2.6.1.42) from rat brain cytosol (BCATc). On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the enzyme appeared as a single band with a molecular mass of 47 kDa; however, gel exclusion chromatography suggested that BCATc is a dimer. Comparison of tryptic peptide maps of BCATc and the mitochondrial form of the enzyme (BCATm) indicated that they are different proteins. Experiments with protein labeling reagents, in particular sulfhydryl reagents, also suggested that there may be some distinct structural differences in BCATc and BCATm. Nevertheless, BCATc and BCATm showed similar specificities for amino acid and alpha-keto acid substrates. Both enzymes transaminated branched chain amino acids, their straight chain analogs, L-alloisoleucine and glutamate. A broader range of alpha-keto acids than amino acids was accepted as substrate including alpha-ketobutyrate and the alpha-keto acid of methionine. Both enzymes exhibited ping-pong kinetics with apparent Km values for leucine and isoleucine of about 1 and 5 mM for valine, respectively. Km values for alpha-ketoglutarate ranged from about 0.6 to 3 mM depending on the amino acid substrate. Polyclonal antibodies were raised in rabbits against purified BCATc. BCATc antiserum neutralized branched chain aminotransferase activity in rat brain cytosol but did not affect the activity in a heart mitochondrial extract. However, immunoblotting showed that BCATc and BCATm do share common epitopes since BCATm antiserum recognized BCATc on the immunoblots. The tissue distribution of BCATc was examined using BCATc and BCATm antisera. These data showed that BCATc was found in adult and fetal rat brain, cultured cells from fetal rat brain cortex, ovary, and placenta. Brain had the highest activity followed by ovary, fetal brain, and placenta. BCATc was not found in fetal liver, adult rat liver, or a rat hepatoma cell line. These data provide clear evidence that BCATc, unlike BCATm, is restricted to several highly specialized tissues." @default.
• W1569754812 created "2016-06-24" @default.
• W1569754812 creator A5023692478 @default.
• W1569754812 creator A5030895139 @default.
• W1569754812 creator A5054773881 @default.
• W1569754812 creator A5072918779 @default.
• W1569754812 date "1993-02-01" @default.
• W1569754812 modified "2023-10-13" @default.
• W1569754812 title "Branched chain aminotransferase isoenzymes. Purification and characterization of the rat brain isoenzyme." @default.
• W1569754812 cites W1487793424 @default.
• W1569754812 cites W1490154674 @default.
• W1569754812 cites W1491920498 @default.
• W1569754812 cites W1494772917 @default.
• W1569754812 cites W1501497173 @default.
• W1569754812 cites W1515213049 @default.
• W1569754812 cites W1603747702 @default.
• W1569754812 cites W1793939457 @default.
• W1569754812 cites W1942398216 @default.
• W1569754812 cites W1967877208 @default.
• W1569754812 cites W1979492674 @default.
• W1569754812 cites W1991259391 @default.
• W1569754812 cites W2001749903 @default.
• W1569754812 cites W2016474954 @default.
• W1569754812 cites W2026406796 @default.
• W1569754812 cites W2059994807 @default.
• W1569754812 cites W2073879684 @default.
• W1569754812 cites W2082497364 @default.
• W1569754812 cites W2084855813 @default.
• W1569754812 cites W2100018141 @default.
• W1569754812 cites W2100837269 @default.
• W1569754812 cites W2111301002 @default.
• W1569754812 cites W2118575411 @default.
• W1569754812 cites W2153334627 @default.
• W1569754812 cites W2176224597 @default.
• W1569754812 cites W2233178365 @default.
• W1569754812 cites W2396226567 @default.
• W1569754812 cites W2397361775 @default.
• W1569754812 cites W2412120034 @default.
• W1569754812 cites W4246090862 @default.
• W1569754812 doi "https://doi.org/10.1016/s0021-9258(18)53663-2" @default.
• W1569754812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8381418" @default.
• W1569754812 hasPublicationYear "1993" @default.
• W1569754812 type Work @default.
• W1569754812 sameAs 1569754812 @default.
• W1569754812 citedByCount "128" @default.
• W1569754812 countsByYear W15697548122012 @default.
• W1569754812 countsByYear W15697548122013 @default.
• W1569754812 countsByYear W15697548122014 @default.
• W1569754812 countsByYear W15697548122015 @default.
• W1569754812 countsByYear W15697548122016 @default.
• W1569754812 countsByYear W15697548122017 @default.
• W1569754812 countsByYear W15697548122018 @default.
• W1569754812 countsByYear W15697548122019 @default.
• W1569754812 countsByYear W15697548122020 @default.
• W1569754812 countsByYear W15697548122021 @default.
• W1569754812 countsByYear W15697548122022 @default.
• W1569754812 countsByYear W15697548122023 @default.
• W1569754812 crossrefType "journal-article" @default.
• W1569754812 hasAuthorship W1569754812A5023692478 @default.
• W1569754812 hasAuthorship W1569754812A5030895139 @default.
• W1569754812 hasAuthorship W1569754812A5054773881 @default.
• W1569754812 hasAuthorship W1569754812A5072918779 @default.
• W1569754812 hasBestOaLocation W15697548121 @default.
• W1569754812 hasConcept C119795356 @default.
• W1569754812 hasConcept C153911025 @default.
• W1569754812 hasConcept C171250308 @default.
• W1569754812 hasConcept C181199279 @default.
• W1569754812 hasConcept C185592680 @default.
• W1569754812 hasConcept C192562407 @default.
• W1569754812 hasConcept C2780841128 @default.
• W1569754812 hasConcept C55493867 @default.
• W1569754812 hasConcept C86803240 @default.
• W1569754812 hasConceptScore W1569754812C119795356 @default.
• W1569754812 hasConceptScore W1569754812C153911025 @default.
• W1569754812 hasConceptScore W1569754812C171250308 @default.
• W1569754812 hasConceptScore W1569754812C181199279 @default.
• W1569754812 hasConceptScore W1569754812C185592680 @default.
• W1569754812 hasConceptScore W1569754812C192562407 @default.
• W1569754812 hasConceptScore W1569754812C2780841128 @default.
• W1569754812 hasConceptScore W1569754812C55493867 @default.
• W1569754812 hasConceptScore W1569754812C86803240 @default.
• W1569754812 hasIssue "5" @default.
• W1569754812 hasLocation W15697548121 @default.
• W1569754812 hasOpenAccess W1569754812 @default.
• W1569754812 hasPrimaryLocation W15697548121 @default.
• W1569754812 hasRelatedWork W1999987807 @default.
• W1569754812 hasRelatedWork W2040812767 @default.
• W1569754812 hasRelatedWork W2050182051 @default.
• W1569754812 hasRelatedWork W2070775953 @default.
• W1569754812 hasRelatedWork W2080141603 @default.
• W1569754812 hasRelatedWork W2080901561 @default.
• W1569754812 hasRelatedWork W2081960730 @default.
• W1569754812 hasRelatedWork W2095539628 @default.
• W1569754812 hasRelatedWork W2356922729 @default.
• W1569754812 hasRelatedWork W613887516 @default.
• W1569754812 hasVolume "268" @default.
• W1569754812 isParatext "false" @default.
• W1569754812 isRetracted "false" @default.

• next