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• W1569756617 abstract "A method to microscopically detect and identify individual cells of members of the domains Bacteria and Archaea is presented. rRNA-targeted oligonucleotides were 5' end labeled with the enzyme horseradish peroxidase and used for whole-cell hybridization. Specifically bound probe was visualized by the enzymatic formation of an intracellular precipitate from the substrate diaminobenzidine. Permeation of the enzyme-labeled probe into whole fixed cells of gram-negative bacteria required their pretreatment with lysozyme-EDTA, whereas permeability of some archaebacterial cells was improved by addition of detergent to the hybridization buffer. Hitherto we had not achieved penetration of enzyme-labeled probe into gram-positive bacteria and yeast cells. This method should be a valuable tool for identification of suitable prokaryotic cells in environments with elevated background fluorescence or in situations in which an epifluorescence microscope is not available." @default.
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• W1569756617 date "1992-09-01" @default.
• W1569756617 modified "2023-10-12" @default.
• W1569756617 title "Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes" @default.
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• W1569756617 doi "https://doi.org/10.1128/aem.58.9.3007-3011.1992" @default.
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