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- W1569763442 abstract "Abstract 151 independent suppressor-negative derivatives of a T4-coded nonsense suppressor, psub+, have been isolated and characterized. They fall into two complementation groups. One, designated mb (modifier of psub+ phenotype), is unlinked to psub+ and has been located about 10 map units from rII. The second group, designated psub−, includes deletions and single base changes which affect sites within 0.2 map units of the original mutation. The secondary mutations in those psub− mutants which still contain the original mutation, psub, have been positioned within the gene for the serine-transfer RNA by a series of two- and threefactor genetic crosses. 32P-labeled tRNA from mb, psub− and wild-type infected cells has been compared by polyacrylamide gel electrophoresis. In mb-infected cells several of the tRNA species are missing, while in psub−-infected cells only the ser-tRNA is clearly absent. These studies suggest there are only two phage genes which are essential for the production of functional ser-tRNA. One is the structural gene for the ser-tRNA and the second plays an undefined role which affects several tRNA's." @default.
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- W1569763442 date "1972-08-01" @default.
- W1569763442 modified "2023-09-26" @default.
- W1569763442 title "Bacteriophage T4 transfer RNA" @default.
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- W1569763442 doi "https://doi.org/10.1016/0022-2836(72)90023-x" @default.
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