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- W1569800580 abstract "A locus involved in the expression of the uptake hydrogenase system of Bradyrhizobium japonicum was identified adjacent to genes encoding the hydrogenase subunits. A cloned fragment of DNA was used to complement to autotrophy a Hup − putative regulatory mutant of B. japonicum . The mutant strain lacked hydrogenase activity and synthesized low levels of the large subunit of hydrogenase as determined by Western gels. Tn 5 -induced mutagenesis located the region within the fragment which was necessary for complementation of the mutant phenotype. The locus identified is adjacent to that encoding the small subunit of hydrogenase; its right border is <0.5 kilobase upstream from the hydrogenase transcriptional start site, and its left border is between 1 and 2.5 kilobases from that start site. However, the locus is outside the region previously shown to contain hup -related genes of B. japonicum . Thus, the localization of this gene describes a previously unidentified hup -related gene on a region of DNA not previously shown to contain hup -specific DNA." @default.
- W1569800580 created "2016-06-24" @default.
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- W1569800580 date "1989-12-01" @default.
- W1569800580 modified "2023-09-26" @default.
- W1569800580 title "Identification of a Locus Upstream from the Hydrogenase Structural Genes That Is Involved in Hydrogenase Expression in Bradyrhizobium japonicum" @default.
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- W1569800580 doi "https://doi.org/10.1128/aem.55.12.3051-3057.1989" @default.
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