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- W1569829656 abstract "Abstract DNA typing has revolutionized forensic biology and has resulted in a dramatic increase in the ability to obtain probative information from crimes involving biological material. Standard typing protocols permit the detection of as little as 100 pg of DNA and therefore may not be able to accommodate low copy number (LCN) samples (defined here as containing <100 pg of template DNA) frequently encountered in forensic casework. In an attempt to increase the recovery of profiles from such LCN samples, whole genome amplification (WGA) strategies have been developed. WGA methods attempt to increase the effective number of starting genomic templates prior to any downstream analysis. Theoretically, by preamplifying the limited amount of genetic material in the sample, sufficient quantities of template can be produced to overcome stochastic effects resulting from LCN templates. We provide an overview of existing WGA methods and describe their applications in forensic DNA analysis. Although WGA methods have yet to demonstrate their utility for routine forensic analysis, selective genomic enrichment and the production of sufficient sample for multiple repeat analyses are possible novel applications." @default.
- W1569829656 created "2016-06-24" @default.
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- W1569829656 date "2009-04-17" @default.
- W1569829656 modified "2023-10-11" @default.
- W1569829656 title "Whole Genome Amplification" @default.
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- W1569829656 doi "https://doi.org/10.1002/9780470061589.fsa542" @default.
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