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- W1569832569 abstract "Regeneration of somatic tissues, and especially gametophytic tissues, by in vitro culture can produce variability. The progressive introduction of sodium chloride during this phase should specifically redirect variability and lead to increased tolerance. Several Moroccan barley (Hordeum vulgare L.) genotypes (Asni, Tamelalt, 905) and a European genotype (Berenice) were thus used in vitro to obtain regeneration through gametophyte culture. From either ovaries or anthers, the “amorphous” calluses only gave rise to albino plantlets or remained non-regenerating, while the “regenerating” calluses gave rise to chlorophyllous plantlets. These latter calluses were transferred to in vitro pressure conditions, with successive chloride sodium concentrations, increasing from 5 to 15 g/l (by 5 g/l) and up to 18 g/l (by 1 g/l), beyond which the conditions were totally lethal. Under control conditions (in vitro medium without stressing agent), 39 fertile plantlets were obtained for all genotypes, producing 346 grains from androgenetic regenerants and 186 from gynogenetic regenerants. Under saline stress conditions, tillers of androgenetic plantlets were often fertile, e.g. Tamelalt and Asni. Following 1-5 transfers on media containing from 5 to 15 g/l sodium chloride, 5 fertile plantlets and a total of 78 grains were obtained. After gynogenesis, tillers of regenerated plants were often sterile, despite colchicine treatment, which might have failed or disturbed the plantlets. Only Berenice, with 4 gynogenetic regenerants derived from 5-15 g/l medium after 1-3 transfers, gave rise to 90 grains. The outcome of this work was 95 fertile plants, regenerated in all conditions, which produced more than 2, 450 grains that will be multiplied for tolerance analysis and verification of hereditary transmission of this potential." @default.
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- W1569832569 date "2000-09-12" @default.
- W1569832569 modified "2023-09-24" @default.
- W1569832569 title "Regeneration by in vitro androgenesis and gynogenesis to obtain somaclonal variation and tolerance to salinity in barley Hordeum vulgare" @default.
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