FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569963265> ?p ?o ?g. }

• W1569963265 abstract "Flagellin, the major subunit of the bacterial motility organ flagellum is an archetypical elicitor molecule perceived by a variety of plant species (Felix et al., 1999). Flg22, a synthetic peptide comprising the highly conserved amino acid residues of the flagellin N-terminus, has been shown to be the active epitope of flagellin which is recognized by plants and sufficient to activate plant defense responses (Felix et al., 1999). Flagellin/flg22 recognition has been attributed to a single protein, FLS2. FLS2 is a leucine rich repeat (LRR) receptor like kinase (RLK), consisting of 28 extracellular LRRs, a single transmembrane domain and an intracellular ser/thr kinase domain, was first identified in the model plant Arabidopsis thaliana (Gomez-Gomez and Boller, 2000) and shown to directly bind flg22 (Chinchilla et al., 2006). Meanwhile, orthologues of FLS2 have been identified in a variety of species from different families, among them tomato (Lycopersicon esculentum), Nicotiana benthamiana, Ricinus communis and Populus trichocarpa, to name just a few (Robatzek et al., 2007b). Although all these plants recognize flg22 as an elicitor, distinct species specific differences were identified. In this work, the molecular differences between the flagellin recognition systems of Arabidopsis (AtFLS2) and tomato (LeFLS2) are analyzed in depth. It was shown that full length flg22 is required for activity in Arabidopsis while tomato requires only the 15 aa peptide flg15 for full stimulation of defense responses (Meindl et al., 2000). Receptor activation of FLS2 by flg22 occurs according to the address-message concept with binding of the address as a first step, and message-induced receptor activation as a second step (Meindl et al., 2000). By using a variety of flg22-derivatives, we analyze how Arabidopsis and tomato flagellin receptors discriminate between different variants of flg22 in terms of binding and receptor activation. By using the species specific differences of Arabidopsis and tomato flagellin perception, we identify areas within the LRR domain of the respective flagellin receptors which are responsible for interaction with the ligand. To achieve this, we constructed a series of chimeric receptors by swapping different parts of the LRR domain from LeFLS into the AtFLS2 sequence. These chimeric receptors were transformed into Nicotiana benthamiana and Arabidopsis thaliana and the transformed plants were tested for receptor function using various bioassays such as ethylene production and growth inhibition and we performed binding assays using immunoprecipitated receptors. Based on these experiments we show that the LeLRR 1 to 10 are sufficient to bind the minimum peptide flg15-Δ7, the shortest flg22-derivative perceived by tomato consisting only of the central 8 amino acids of flg22. We show that the initial ten N-terminal LRRs between the amino acids 32-337 and within this area, especially the amino acids 236-337 are import for the higher affinity of LeFLS2 to flg22 and N-terminally truncated flg22-derivatives. We further show that an additional region between the LRR 19 to 24 of LeFLS2 is involved in the recognition of the C-terminus of flg22. Because the C-terminus of flg22 has been shown to be part of the “message”, which activates receptor signaling (Meindl et al., 2000; Chinchilla et al., 2006), we propose the region of LRR 19 to 24 to be play an important role for activation of FLS2. Additionally achimeric receptor between AtFLS2 and LeFLS2 is presented which shows the characteristics of a constitutive active FLS2 allele when transformed into Arabidopsis. Interestingly, the constitutive signaling of this chimeric receptor can only be triggered via the artificial extracellular LRR domain, since the complete intracellular receptor part, e.g. transmembrane- juxtamembrane- and kinasedomain is not affected from the LRR domain swapping. Together, this study provides new insight towards the understanding of FLS2-ligand interaction and an interesting tool to further study receptor activation." @default.
• W1569963265 created "2016-06-24" @default.
• W1569963265 creator A5003518187 @default.
• W1569963265 date "2010-01-01" @default.
• W1569963265 modified "2023-09-24" @default.
• W1569963265 title "Innate immunity mediated by the flagellin receptor FLS2 in Arabidopsis and tomato: A molecular approach to characterize ligand binding and function, using receptor chimeras" @default.
• W1569963265 cites W1491616831 @default.
• W1569963265 cites W1493172387 @default.
• W1569963265 cites W1496461152 @default.
• W1569963265 cites W1514065503 @default.
• W1569963265 cites W1519633296 @default.
• W1569963265 cites W1571910978 @default.
• W1569963265 cites W1581562089 @default.
• W1569963265 cites W1594203818 @default.
• W1569963265 cites W1636824184 @default.
• W1569963265 cites W1826812957 @default.
• W1569963265 cites W1855952875 @default.
• W1569963265 cites W1925288771 @default.
• W1569963265 cites W1963990261 @default.
• W1569963265 cites W1966651071 @default.
• W1569963265 cites W1967095747 @default.
• W1569963265 cites W1969072247 @default.
• W1569963265 cites W1970216382 @default.
• W1569963265 cites W1970705775 @default.
• W1569963265 cites W1970985233 @default.
• W1569963265 cites W1971799655 @default.
• W1569963265 cites W1973328229 @default.
• W1569963265 cites W1973891844 @default.
• W1569963265 cites W1974664442 @default.
• W1569963265 cites W1975032025 @default.
• W1569963265 cites W1975973064 @default.
• W1569963265 cites W1981045929 @default.
• W1569963265 cites W1987463569 @default.
• W1569963265 cites W1987535382 @default.
• W1569963265 cites W1989413225 @default.
• W1569963265 cites W1989628785 @default.
• W1569963265 cites W1989656760 @default.
• W1569963265 cites W1991001025 @default.
• W1569963265 cites W1991162607 @default.
• W1569963265 cites W1991464867 @default.
• W1569963265 cites W1994391392 @default.
• W1569963265 cites W1994481770 @default.
• W1569963265 cites W1994602890 @default.
• W1569963265 cites W2000350495 @default.
• W1569963265 cites W2001697409 @default.
• W1569963265 cites W2003647281 @default.
• W1569963265 cites W2005626023 @default.
• W1569963265 cites W2006990207 @default.
• W1569963265 cites W2007608885 @default.
• W1569963265 cites W2010321398 @default.
• W1569963265 cites W2013601771 @default.
• W1569963265 cites W2014081607 @default.
• W1569963265 cites W2014602273 @default.
• W1569963265 cites W2014679946 @default.
• W1569963265 cites W2018317190 @default.
• W1569963265 cites W2019854769 @default.
• W1569963265 cites W2026320444 @default.
• W1569963265 cites W2026500006 @default.
• W1569963265 cites W2027364010 @default.
• W1569963265 cites W2027983878 @default.
• W1569963265 cites W2030904208 @default.
• W1569963265 cites W2031609964 @default.
• W1569963265 cites W2033138760 @default.
• W1569963265 cites W2033883110 @default.
• W1569963265 cites W2040024879 @default.
• W1569963265 cites W2040210770 @default.
• W1569963265 cites W2040921242 @default.
• W1569963265 cites W2044873849 @default.
• W1569963265 cites W2046718786 @default.
• W1569963265 cites W2049033930 @default.
• W1569963265 cites W2049886969 @default.
• W1569963265 cites W2050280736 @default.
• W1569963265 cites W2050607290 @default.
• W1569963265 cites W2053246453 @default.
• W1569963265 cites W2053429123 @default.
• W1569963265 cites W2054766392 @default.
• W1569963265 cites W2058448508 @default.
• W1569963265 cites W2059856607 @default.
• W1569963265 cites W2059971395 @default.
• W1569963265 cites W2061085465 @default.
• W1569963265 cites W2064419492 @default.
• W1569963265 cites W2064626913 @default.
• W1569963265 cites W2066743397 @default.
• W1569963265 cites W2067736347 @default.
• W1569963265 cites W2068307748 @default.
• W1569963265 cites W2069502810 @default.
• W1569963265 cites W2069981713 @default.
• W1569963265 cites W2072425598 @default.
• W1569963265 cites W2073376866 @default.
• W1569963265 cites W2075130941 @default.
• W1569963265 cites W2075583035 @default.
• W1569963265 cites W2075948284 @default.
• W1569963265 cites W2076361525 @default.
• W1569963265 cites W2077074452 @default.
• W1569963265 cites W2077404754 @default.
• W1569963265 cites W2079209641 @default.
• W1569963265 cites W2081110616 @default.
• W1569963265 cites W2082890018 @default.
• W1569963265 cites W2084484077 @default.
• W1569963265 cites W2084858774 @default.

• next