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- W1570075445 abstract "This chapter discusses the visual assay for chromosome ploidy. The chapter describes the ade3-2p and SUPII visual assays for following changes in chromosome ploidy during mitotic divisions of S.cerevisiae. The chapter presents three experimental applications of the assays: the sectoring assay, a qualitative assay for identifying cis and trans mutations that show an altered fidelity of chromosome transmission; fluctuation analysis, a quantitative method for determining changes in the rate of chromosome loss or gain; and half-sectoring colony analysis, an assay for determining the types of aberrant transmissions that are responsible for producing cells with altered chromosome ploidy. The chapter describes the general principles and the materials and reagents necessary to perform each application and the sample experiments, which demonstrate their use and limitations in the isolation and characterization of mutants affecting chromosome transmission. The sample experiments use the ade3-2p system." @default.
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- W1570075445 date "1987-01-01" @default.
- W1570075445 modified "2023-10-09" @default.
- W1570075445 title "[22] Visual assay for chromosome ploidy" @default.
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- W1570075445 doi "https://doi.org/10.1016/0076-6879(87)55024-8" @default.
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