Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570209659> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1570209659 endingPage "256" @default.
- W1570209659 startingPage "246" @default.
- W1570209659 abstract "Neutrophils contain phospholipase D, which is activated by receptor-coupled agonists, such as f-Met–Leu–Phe and protein kinase C activators, such as phorbol esters. Phospholipase D catalyzes the hydrolysis of phospholipids into phosphatidic acid, and it has been implicated in signal transduction in the neutrophil as well as in a variety of other cells. Neutrophils are isolated based on the procedure by Pember et al. In this procedure, blood (450 ml) is obtained by venapuncture in a 600-mL transfer pack container containing 10 mL of 0.1 M EDTA, pH 7.4/0.9% of NaCl. Blood is then diluted with 180-mL Hespan and is allowed to settle in plastic cylinders for one hour at room temperature. At no time during the preparation are cells allowed to come into contact with glass. The chapter explains the labeling of cells and isolation of membrane and cytosolic fractions. A variety of assays are available for measuring phospholipase D activity, which include the formation of labeled phosphatidic acid, the release of a labeled choline headgroup, and transphosphatidylation." @default.
- W1570209659 created "2016-06-24" @default.
- W1570209659 creator A5027564508 @default.
- W1570209659 creator A5049024406 @default.
- W1570209659 creator A5079957920 @default.
- W1570209659 date "1995-01-01" @default.
- W1570209659 modified "2023-09-24" @default.
- W1570209659 title "[27] Neutrophil phospholipase D: Inhibition by Rho-GDP dissociation inhibitor and stimulation by small GTPase GDP dissociation stimulator" @default.
- W1570209659 cites W1493531388 @default.
- W1570209659 cites W1528198451 @default.
- W1570209659 cites W1528319719 @default.
- W1570209659 cites W1528720934 @default.
- W1570209659 cites W1558257735 @default.
- W1570209659 cites W1560104562 @default.
- W1570209659 cites W1805425496 @default.
- W1570209659 cites W2053057329 @default.
- W1570209659 cites W2060679254 @default.
- W1570209659 cites W2152311269 @default.
- W1570209659 cites W2402400379 @default.
- W1570209659 cites W250899876 @default.
- W1570209659 cites W2804275696 @default.
- W1570209659 doi "https://doi.org/10.1016/0076-6879(95)56029-7" @default.
- W1570209659 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7476438" @default.
- W1570209659 hasPublicationYear "1995" @default.
- W1570209659 type Work @default.
- W1570209659 sameAs 1570209659 @default.
- W1570209659 citedByCount "5" @default.
- W1570209659 crossrefType "book-chapter" @default.
- W1570209659 hasAuthorship W1570209659A5027564508 @default.
- W1570209659 hasAuthorship W1570209659A5049024406 @default.
- W1570209659 hasAuthorship W1570209659A5079957920 @default.
- W1570209659 hasConcept C12927208 @default.
- W1570209659 hasConcept C134018914 @default.
- W1570209659 hasConcept C180373561 @default.
- W1570209659 hasConcept C181199279 @default.
- W1570209659 hasConcept C185592680 @default.
- W1570209659 hasConcept C24998067 @default.
- W1570209659 hasConcept C2776909261 @default.
- W1570209659 hasConcept C2778597717 @default.
- W1570209659 hasConcept C2778918659 @default.
- W1570209659 hasConcept C41625074 @default.
- W1570209659 hasConcept C55493867 @default.
- W1570209659 hasConcept C6182249 @default.
- W1570209659 hasConcept C62478195 @default.
- W1570209659 hasConcept C71240020 @default.
- W1570209659 hasConcept C86803240 @default.
- W1570209659 hasConceptScore W1570209659C12927208 @default.
- W1570209659 hasConceptScore W1570209659C134018914 @default.
- W1570209659 hasConceptScore W1570209659C180373561 @default.
- W1570209659 hasConceptScore W1570209659C181199279 @default.
- W1570209659 hasConceptScore W1570209659C185592680 @default.
- W1570209659 hasConceptScore W1570209659C24998067 @default.
- W1570209659 hasConceptScore W1570209659C2776909261 @default.
- W1570209659 hasConceptScore W1570209659C2778597717 @default.
- W1570209659 hasConceptScore W1570209659C2778918659 @default.
- W1570209659 hasConceptScore W1570209659C41625074 @default.
- W1570209659 hasConceptScore W1570209659C55493867 @default.
- W1570209659 hasConceptScore W1570209659C6182249 @default.
- W1570209659 hasConceptScore W1570209659C62478195 @default.
- W1570209659 hasConceptScore W1570209659C71240020 @default.
- W1570209659 hasConceptScore W1570209659C86803240 @default.
- W1570209659 hasLocation W15702096591 @default.
- W1570209659 hasLocation W15702096592 @default.
- W1570209659 hasOpenAccess W1570209659 @default.
- W1570209659 hasPrimaryLocation W15702096591 @default.
- W1570209659 hasRelatedWork W1447066740 @default.
- W1570209659 hasRelatedWork W1540777723 @default.
- W1570209659 hasRelatedWork W1569240903 @default.
- W1570209659 hasRelatedWork W1987606489 @default.
- W1570209659 hasRelatedWork W2072776814 @default.
- W1570209659 hasRelatedWork W2272683735 @default.
- W1570209659 hasRelatedWork W2323321380 @default.
- W1570209659 hasRelatedWork W2418568965 @default.
- W1570209659 hasRelatedWork W4289528401 @default.
- W1570209659 hasRelatedWork W4385980586 @default.
- W1570209659 isParatext "false" @default.
- W1570209659 isRetracted "false" @default.
- W1570209659 magId "1570209659" @default.
- W1570209659 workType "book-chapter" @default.