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- W1570245607 abstract "Escherichia coli grown for many generations in 18O-enriched water shows a steady state 18O content of the γ-phosphoryl group of intracellular ATP approximately 70% of that in the medium water. This 18O labeling pattern is similar to the one previously observed in Bacillus subtilis (Duffy, Chaney & Boyer, 1972). However in E. coli the steady-state 18O level of the phosphodiester branch oxygens of the RNA is significantly higher than the γ-phosphoryl of ATP, indicating the introduction of an extra source of water oxygens through hydrolytic turnover of the RNA. The results give evidence that the dominant pathway of unstable RNA degradation in E. coli is catalyzed by hydrolytic and not by phosphorolytic enzymes. The data also allow estimation that unstable RNA is synthesized approximately 2.6 times as fast as stable RNA under conditions used. Thus, 72% of the RNA synthesized at any time would be unstable. This estimate is somewhat higher than previous estimates by different procedures." @default.
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- W1570245607 date "1972-03-01" @default.
- W1570245607 modified "2023-10-17" @default.
- W1570245607 title "Incorporation of water oxygens into intracellular nucleotides and RNA" @default.
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- W1570245607 doi "https://doi.org/10.1016/0022-2836(72)90084-8" @default.
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