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• W1570252009 abstract "Prostate cancer is the most common form of cancer amongst males in the western world. Today’s diagnostic methods for prostate cancer that rely on PSA measurement are not satisfying. The level of prostate specific antigen (PSA) is determined in a blood test. Of the men with elevated PSA levels only 25 % have prostate cancer. This thesis aims to develop a proof of concept for a miniaturized multiplex microarray, which in a future perspective could be used as a diagnostic tool for prostate cancer. Different diagnostic methods are used today but none are separately sufficiently specific. The microarray based method in this thesis aims to a screening technique for simultaneous measurements of total, free and intact PSA as well as free human kallikrein 2 (hK2) in serum. The intermediate goal was a dual approach where PSA total and free are measured on two chips. The dual assay paved the way to the multiplex approach where different biomarkers simultaneously were measured on the same porous silicon chip. In both approaches a capture antibody was dispensed onto a porous surface. The chip was incubated with the biological sample and detected with a fluorescently labeled detector antibody in a confocal microscope. The intermediate dual assay was validated using 8 plasma samples obtained from the clinical routine analysis. Furthermore, three biomarkers (PSA total, free and intact) were successfully detected simultaneously on a porous silicon chip sized 3.5 x 3.5 mm." @default.
• W1570252009 created "2016-06-24" @default.
• W1570252009 creator A5083434247 @default.
• W1570252009 date "2009-01-01" @default.
• W1570252009 modified "2023-09-27" @default.
• W1570252009 title "Miniaturized Multiplex Approaches for Prostate Cancer Diagnostics - Detection of hK2 and Various Forms of PSA" @default.
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