Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570288743> ?p ?o ?g. }
- W1570288743 endingPage "1435" @default.
- W1570288743 startingPage "1426" @default.
- W1570288743 abstract "J. Neurochem. (2010) 113 , 1426–1435. Abstract The glutamate transporters EAAT3 and EAAT4 are expressed in neurons. They contribute to the cellular uptake of glutamate and aspartate and thus to the clearance of the excitatory transmitters from the extracellular space. During ischemia, extracellular accumulation of glutamate may trigger excitotoxicity. Energy depletion leads to activation of the AMP‐activated protein kinase (AMPK), a kinase enhancing energy production and limiting energy expenditure. The present study thus explored the possibility that AMPK regulates EAAT3 and/or EAAT4. To this end, EAAT3 or EAAT4 were expressed in Xenopus oocytes with or without AMPK and electrogenic glutamate transport determined by dual electrode voltage clamp. In EAAT3‐ and in EAAT4‐expressing oocytes glutamate generated a current ( I g ), which was half maximal ( K M ) at 74 μM (EAAT3) or at 4 μM (EAAT4) glutamate. Co‐expression of constitutively active γR70Q AMPK or of wild type AMPK did not affect K M but significantly decreased the maximal I g in both EAAT3‐ (by 34%) and EAAT4‐ (by 49%) expressing oocytes. Co‐expression of the inactive mutant αK45R AMPK [α1(K45R)β1γ1] did not appreciably affect I g . According to confocal microscopy and chemiluminescence co‐expression of γR70Q AMPK or of wild type AMPK reduced the membrane abundance of EAAT3 and EAAT4. The observations show that AMPK down‐regulates Na + ‐coupled glutamate transport." @default.
- W1570288743 created "2016-06-24" @default.
- W1570288743 creator A5004202808 @default.
- W1570288743 creator A5025098806 @default.
- W1570288743 creator A5026423976 @default.
- W1570288743 creator A5048663566 @default.
- W1570288743 creator A5061162593 @default.
- W1570288743 creator A5061636268 @default.
- W1570288743 creator A5084208566 @default.
- W1570288743 date "2010-06-01" @default.
- W1570288743 modified "2023-10-16" @default.
- W1570288743 title "Down-regulation of Na+-coupled glutamate transporter EAAT3 and EAAT4 by AMP-activated protein kinase" @default.
- W1570288743 cites W1271531431 @default.
- W1570288743 cites W1502922710 @default.
- W1570288743 cites W1538613429 @default.
- W1570288743 cites W1592654973 @default.
- W1570288743 cites W1811953508 @default.
- W1570288743 cites W1827729646 @default.
- W1570288743 cites W1959374451 @default.
- W1570288743 cites W1966605194 @default.
- W1570288743 cites W1967155114 @default.
- W1570288743 cites W1969826464 @default.
- W1570288743 cites W1970919546 @default.
- W1570288743 cites W1971752070 @default.
- W1570288743 cites W1976854136 @default.
- W1570288743 cites W1979803421 @default.
- W1570288743 cites W1980114617 @default.
- W1570288743 cites W1983563653 @default.
- W1570288743 cites W1983943582 @default.
- W1570288743 cites W1985161328 @default.
- W1570288743 cites W1987520241 @default.
- W1570288743 cites W1988848089 @default.
- W1570288743 cites W1997560040 @default.
- W1570288743 cites W1998846464 @default.
- W1570288743 cites W2005329028 @default.
- W1570288743 cites W2019535483 @default.
- W1570288743 cites W2020620553 @default.
- W1570288743 cites W2026519847 @default.
- W1570288743 cites W2028227113 @default.
- W1570288743 cites W2034042784 @default.
- W1570288743 cites W2034878114 @default.
- W1570288743 cites W2036009366 @default.
- W1570288743 cites W2037354491 @default.
- W1570288743 cites W2037414045 @default.
- W1570288743 cites W2045609345 @default.
- W1570288743 cites W2053050620 @default.
- W1570288743 cites W2057998788 @default.
- W1570288743 cites W2061978202 @default.
- W1570288743 cites W2062109867 @default.
- W1570288743 cites W2063619823 @default.
- W1570288743 cites W2066161326 @default.
- W1570288743 cites W2069811002 @default.
- W1570288743 cites W2070727719 @default.
- W1570288743 cites W2072314593 @default.
- W1570288743 cites W2073953555 @default.
- W1570288743 cites W2074558304 @default.
- W1570288743 cites W2078083464 @default.
- W1570288743 cites W2078517229 @default.
- W1570288743 cites W2082190932 @default.
- W1570288743 cites W2084240121 @default.
- W1570288743 cites W2088134325 @default.
- W1570288743 cites W2092939335 @default.
- W1570288743 cites W2095539444 @default.
- W1570288743 cites W2096675020 @default.
- W1570288743 cites W2099032741 @default.
- W1570288743 cites W2113877948 @default.
- W1570288743 cites W2119774379 @default.
- W1570288743 cites W2119997506 @default.
- W1570288743 cites W2120668034 @default.
- W1570288743 cites W2123016546 @default.
- W1570288743 cites W2123397214 @default.
- W1570288743 cites W2124966591 @default.
- W1570288743 cites W2125428479 @default.
- W1570288743 cites W2127941025 @default.
- W1570288743 cites W2131855154 @default.
- W1570288743 cites W2135424721 @default.
- W1570288743 cites W2150551377 @default.
- W1570288743 cites W2158080606 @default.
- W1570288743 cites W2161452179 @default.
- W1570288743 cites W2162776870 @default.
- W1570288743 cites W2164134117 @default.
- W1570288743 cites W2169384785 @default.
- W1570288743 cites W2169983816 @default.
- W1570288743 cites W2176414067 @default.
- W1570288743 cites W2224021986 @default.
- W1570288743 cites W2915443674 @default.
- W1570288743 cites W4237590128 @default.
- W1570288743 doi "https://doi.org/10.1111/j.1471-4159.2010.06678.x" @default.
- W1570288743 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20218975" @default.
- W1570288743 hasPublicationYear "2010" @default.
- W1570288743 type Work @default.
- W1570288743 sameAs 1570288743 @default.
- W1570288743 citedByCount "12" @default.
- W1570288743 countsByYear W15702887432012 @default.
- W1570288743 countsByYear W15702887432015 @default.
- W1570288743 countsByYear W15702887432018 @default.
- W1570288743 countsByYear W15702887432019 @default.
- W1570288743 countsByYear W15702887432020 @default.