Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570311509> ?p ?o ?g. }
- W1570311509 endingPage "305" @default.
- W1570311509 startingPage "289" @default.
- W1570311509 abstract "Cationic lipids (CLs) are being increasingly exploited as transfection vectors for the delivery of DNA into eukaryotic cells. To obtain further insight to the complex formation and interactions between cationic liposomes and DNA, we characterised three novel cationic lipids, viz. bis[2-(11-phenoxyundecanoate)ethyl]-dimethylammonium bromide, N-hexadecyl-N-¿10-[O-(4-acetoxy)-phenylundecanoate]ethyl¿- dimethylammonium bromide, and bis[2-(11-butyloxyundecanoate)ethyl]dimethylammonium bromide. These lipids bear the same charged headgroup yet have different hydrophobic parts. Accordingly, we may anticipate their electrostatic interactions with DNA to be similar while differing in both thermal phase behaviour and physicochemical properties of their complexes with DNA. In keeping with the above all three lipids formed complexes with DNA as evidenced by light scattering, fluorescence spectroscopy and Langmuir film balance. Differential scanning calorimetry revealed very different phase behaviours for the binary mixtures of the three CLs with dimyristoylphosphatidylcholine and also provided evidence for DNA-induced lipid phase separation. These data were confirmed by compression isotherms and fluorescence microscopy of monolayers residing on an aqueous buffer, recorded both in the presence and absence of DNA. Importantly, binding to cationic liposomes appears to prevent thermal denaturation of DNA upon heating of the complexes. Likewise, renaturation of heat-treated DNA complexed with the cationic liposomes appears to be abolished as well." @default.
- W1570311509 created "2016-06-24" @default.
- W1570311509 creator A5008795187 @default.
- W1570311509 creator A5009637284 @default.
- W1570311509 creator A5078596818 @default.
- W1570311509 creator A5084274768 @default.
- W1570311509 creator A5088392007 @default.
- W1570311509 creator A5091801332 @default.
- W1570311509 date "2000-06-01" @default.
- W1570311509 modified "2023-10-18" @default.
- W1570311509 title "Characterisation of three novel cationic lipids as liposomal complexes with DNA11A preliminary account of the key findings of this study was communicated in the 25th FEBS 98 Silver Jubilee Meeting, July 5–10, 1998, held in Copenhagen, Denmark." @default.
- W1570311509 cites W1275407085 @default.
- W1570311509 cites W1454209 @default.
- W1570311509 cites W1513897103 @default.
- W1570311509 cites W1542117563 @default.
- W1570311509 cites W1579604458 @default.
- W1570311509 cites W1965522811 @default.
- W1570311509 cites W1968232819 @default.
- W1570311509 cites W1969898499 @default.
- W1570311509 cites W1971290197 @default.
- W1570311509 cites W1972655112 @default.
- W1570311509 cites W1983351621 @default.
- W1570311509 cites W1984441606 @default.
- W1570311509 cites W1985701428 @default.
- W1570311509 cites W1986364564 @default.
- W1570311509 cites W1987827192 @default.
- W1570311509 cites W1988537267 @default.
- W1570311509 cites W1992156570 @default.
- W1570311509 cites W1994034532 @default.
- W1570311509 cites W1995210301 @default.
- W1570311509 cites W1995789414 @default.
- W1570311509 cites W1997568955 @default.
- W1570311509 cites W1997969933 @default.
- W1570311509 cites W1999373563 @default.
- W1570311509 cites W1999598401 @default.
- W1570311509 cites W2002025577 @default.
- W1570311509 cites W2005262643 @default.
- W1570311509 cites W2014065789 @default.
- W1570311509 cites W2014200281 @default.
- W1570311509 cites W2017553712 @default.
- W1570311509 cites W2022591310 @default.
- W1570311509 cites W2023472552 @default.
- W1570311509 cites W2032234735 @default.
- W1570311509 cites W2032631686 @default.
- W1570311509 cites W2034342858 @default.
- W1570311509 cites W2036925843 @default.
- W1570311509 cites W2038467456 @default.
- W1570311509 cites W2040330223 @default.
- W1570311509 cites W2041153298 @default.
- W1570311509 cites W2043367471 @default.
- W1570311509 cites W2045101135 @default.
- W1570311509 cites W2046232525 @default.
- W1570311509 cites W2046358301 @default.
- W1570311509 cites W2048493801 @default.
- W1570311509 cites W2052793555 @default.
- W1570311509 cites W2053600687 @default.
- W1570311509 cites W2054688134 @default.
- W1570311509 cites W2058445944 @default.
- W1570311509 cites W2059073565 @default.
- W1570311509 cites W2065051465 @default.
- W1570311509 cites W2065073846 @default.
- W1570311509 cites W2066380073 @default.
- W1570311509 cites W2073537725 @default.
- W1570311509 cites W2075759218 @default.
- W1570311509 cites W2079512015 @default.
- W1570311509 cites W2080648192 @default.
- W1570311509 cites W2082859968 @default.
- W1570311509 cites W2083312272 @default.
- W1570311509 cites W2086274688 @default.
- W1570311509 cites W2089799214 @default.
- W1570311509 cites W2092770422 @default.
- W1570311509 cites W2094029674 @default.
- W1570311509 cites W2099559490 @default.
- W1570311509 cites W2104252046 @default.
- W1570311509 cites W2141778672 @default.
- W1570311509 cites W2143875501 @default.
- W1570311509 cites W2168309632 @default.
- W1570311509 cites W266995794 @default.
- W1570311509 cites W2949870653 @default.
- W1570311509 doi "https://doi.org/10.1016/s0005-2736(00)00183-8" @default.
- W1570311509 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10825450" @default.
- W1570311509 hasPublicationYear "2000" @default.
- W1570311509 type Work @default.
- W1570311509 sameAs 1570311509 @default.
- W1570311509 citedByCount "31" @default.
- W1570311509 countsByYear W15703115092012 @default.
- W1570311509 countsByYear W15703115092016 @default.
- W1570311509 countsByYear W15703115092017 @default.
- W1570311509 crossrefType "journal-article" @default.
- W1570311509 hasAuthorship W1570311509A5008795187 @default.
- W1570311509 hasAuthorship W1570311509A5009637284 @default.
- W1570311509 hasAuthorship W1570311509A5078596818 @default.
- W1570311509 hasAuthorship W1570311509A5084274768 @default.
- W1570311509 hasAuthorship W1570311509A5088392007 @default.
- W1570311509 hasAuthorship W1570311509A5091801332 @default.
- W1570311509 hasBestOaLocation W15703115091 @default.
- W1570311509 hasConcept C104317684 @default.
- W1570311509 hasConcept C121332964 @default.