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- W1570312114 abstract "A standardized protocol for testing feline viral rhinotracheitis is given. Stock virus is best held in freeze-dried aliquots at −5°C. for up to 1 year, and diluted when required in tissue culture medium, supplemented with 10 per cent. heat-inactivated calf serum, to contain 103 TCID50/ml. Test sera, heat-inactivated at 56°C. for 1 hour and stored at −5°C. are serially diluted by 2-fold steps and mixed in equal volumes with diluted virus suspension. Incubation proceeds at 37°C. for 1 hour and overnight (18 hours approximately) at 4°C. Subsequently, 0·2 ml. of serum/virus mixtures are inoculated into feline cell monolayers or suspensions using 2 tubes per dilution and incubated at 37°C. The test is read at approximately 3 days when a negative serum control shows a maximum CPE, and endpoints are calculated as the final dilution of serum at which 50 per cent. of inoculated monolayers would show complete neutralization of virus. A known reference serum is tested simultaneously." @default.
- W1570312114 created "2016-06-24" @default.
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- W1570312114 date "1969-07-01" @default.
- W1570312114 modified "2023-10-11" @default.
- W1570312114 title "A standardized serum neutralization test for feline viral rhinotracheitis" @default.
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- W1570312114 doi "https://doi.org/10.1016/0021-9975(69)90055-3" @default.
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