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- W1570376075 abstract "Abstract Sample prefractionation is essential for more comprehensive coverage and reliable detection of low‐abundance proteins in complex proteomes. An efficient and reproducible new method for sample prefractionation is microscale solution isoelectrofocusing (MicroSol‐IEF), in which samples are separated into chambers defined by membranes of specific pH, yielding well resolved fractions on the basis of isoelectric point (pI). The output seamlessly interfaces with narrow‐pH‐range 2‐D gels, enhancing data obtained from protein profiling studies, including quantitative proteome comparisons. This unit presents the MicroSol‐IEF method using the ZOOM IEF Fractionator with either commercially available or custom‐made pH partition membranes. Alternative configurations are possible for separating samples into different numbers of fractions with various pH ranges and volumes. A detailed method is provided for preparing custom pH membranes. In addition, methods are provided for evaluating the effectiveness of the prefractionation, using 1‐D and 2‐D gel electrophoresis. Approaches for quantitative protein profiling that incorporate MicroSol‐IEF are also discussed." @default.
- W1570376075 created "2016-06-24" @default.
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- W1570376075 date "2005-02-01" @default.
- W1570376075 modified "2023-10-03" @default.
- W1570376075 title "Protein Profiling by Microscale Solution Isoelectrofocusing (MicroSol‐IEF)" @default.
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- W1570376075 doi "https://doi.org/10.1002/0471140864.ps2206s39" @default.
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