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- W1570392362 abstract "以大肠杆菌O157 rfbE和stx2为待检靶基因，设计两对引物和两条MGB探针，rfbE和stx2探针5’端分别用FAM和VIC基团标记，3’端均用Taqman—MGB标记。建立并优化了检测大肠杆菌O157的二重荧光定量PCR方法，可检测的最低DNA浓度是10拷贝／μL；实验中O157菌株检测结果均为rfbE阳性，而非O157菌株检测结果均为阴性；重复性实验中，批间差异小于80％，批内差异小于70％。实验结果显示此二重荧光定量PCR方法可对分离的可疑大肠杆菌O157菌株进行快速鉴定，同时得知菌株是否携带stx2毒力基因，有利于菌株毒力强弱的判定。" @default.
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- W1570392362 date "2007-01-01" @default.
- W1570392362 modified "2023-09-23" @default.
- W1570392362 title "MGB（Minor Groove Binder）复合探针二重实时定量PCR检测大肠杆菌O157" @default.
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