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• W1570421297 abstract "Sendai virus (SV) glycoproteins were co-reconstituted with the erythrocyte membrane polypeptide designated as band 3 (B3). Fusion of the hybrid vesicles, SV-B3, with L. HeLa and Friend erythroleukemic (FEL) cells led to fusion-mediated implantation of the erythrocyte band 3 into the plasma membranes of the above cells. By the use of radiolabelled band 3 ([3H]H2DIDS-B3) it was possible to demonstrate that about 1–4 × 106 molecules of band 3 can be transferred to the membranes of each of the above cell lines. Cultivation of the recipient cells in growth medium resulted in gradual decrease of the cell-associated counts. The reduction pattern followed first-order kinetics, reaching t12 of disappearance at about 25–30 h in the case of L and HeLa cells, and 57 h in the case of FEL cells. NH4Cl added to the growth medium significantly inhibited the disappearance of band 3 during growth. The studies of the present work indicate that the reduction in the cell-associated band 3 during growth may represent degradation of this polypeptide by the recipient cells." @default.
• W1570421297 created "2016-06-24" @default.
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• W1570421297 date "1983-10-01" @default.
• W1570421297 modified "2023-10-16" @default.
• W1570421297 title "Fusion-mediated implantation of band 3 into living cells" @default.
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• W1570421297 doi "https://doi.org/10.1016/0014-4827(83)90190-8" @default.
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