FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W157044733> ?p ?o ?g. }

• W157044733 endingPage "2004" @default.
• W157044733 startingPage "1996" @default.
• W157044733 abstract "A bioluminescent enzyme immunoassay (BEIA), using Salmonella-specific monoclonal antibody M183 for capture and biotinylated monoclonal antibody M183 for detection, was developed with InteLite AB streptavidin-biotinylated firefly luciferase complex as a reporter. Salmonella cultures were preenriched in buffered peptone water with shaking for 6 h at 37 degrees C and then selectively enriched in Muller-Kauffmann tetrathionate (MKTT) broth and modified semisolid Rappaport-Vassiliadis (MSRV) medium for 16 h at 42 degrees C. After enrichment, the total test time for the BEIA was 1.5 h. The analytical sensitivity of the BEIA ranged from 6.0 x 10(2) CFU/ml to 1.2 x 10(5) CFU/ml in MKTT and from 1.4 x 10(5) to 2.3 x 10(6) CFU/ml in MSRV using six Salmonella serovars prevalent in Canada. With enrichment cultures, the BEIA detected 1 CFU of Salmonella Typhimurium and Salmonella Enteritidis in 25 ml of chicken rinses. Representative strains of 10 Salmonella serovars were detected, and cross-reactivity was not observed with 25 non-Salmonella foodborne bacteria. The BEIA performance was assessed by testing 420 poultry samples, which were analyzed in parallel with the standard MSRV culture method. The BEIA detected 117 (27.88%) Salmonella-positive samples, whereas the standard MSRV culture method detected 124 (29.5%). The BEIA had a sensitivity of 64.5% and a specificity of 87.5% compared to the standard MSRV culture method. However, similar specificities and sensitivities were obtained when the standard MSRV culture method was compared to the BEIA (sensitivity = 68.4% and specificity = 85.5%). Neither method detected 100% of the Salmonella found in the samples tested, and statistical analyses indicated no significant difference between the two methods. In summary, the BEIA offers another alternative for the detection of Salmonella, with the additional advantage of providing a 24-h test for detecting Salmonella in chicken carcass rinses. The results obtained in this research indicate that tests are still needed for the isolation and detection of Salmonella that will establish the true prevalence of Salmonella in chicken samples." @default.
• W157044733 created "2016-06-24" @default.
• W157044733 creator A5004282838 @default.
• W157044733 creator A5009018617 @default.
• W157044733 creator A5030350457 @default.
• W157044733 creator A5042490200 @default.
• W157044733 creator A5060600247 @default.
• W157044733 date "2003-11-01" @default.
• W157044733 modified "2023-10-16" @default.
• W157044733 title "Evaluation of a 24-Hour Bioluminescent Enzyme Immunoassay for the Rapid Detection of Salmonella in Chicken Carcass Rinses" @default.
• W157044733 cites W1555271405 @default.
• W157044733 cites W1574773245 @default.
• W157044733 cites W1913974792 @default.
• W157044733 cites W1970307455 @default.
• W157044733 cites W1986812421 @default.
• W157044733 cites W2011212109 @default.
• W157044733 cites W2011332517 @default.
• W157044733 cites W2024163580 @default.
• W157044733 cites W2031165031 @default.
• W157044733 cites W2041791307 @default.
• W157044733 cites W2041800251 @default.
• W157044733 cites W2041824658 @default.
• W157044733 cites W2060332827 @default.
• W157044733 cites W2082330489 @default.
• W157044733 cites W2092493523 @default.
• W157044733 cites W2096278839 @default.
• W157044733 cites W2103812154 @default.
• W157044733 cites W2150424208 @default.
• W157044733 cites W2163523235 @default.
• W157044733 cites W2330616103 @default.
• W157044733 cites W2487669771 @default.
• W157044733 cites W2558802307 @default.
• W157044733 cites W2559490286 @default.
• W157044733 cites W4250996632 @default.
• W157044733 cites W82897896 @default.
• W157044733 doi "https://doi.org/10.4315/0362-028x-66.11.1996" @default.
• W157044733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14627274" @default.
• W157044733 hasPublicationYear "2003" @default.
• W157044733 type Work @default.
• W157044733 sameAs 157044733 @default.
• W157044733 citedByCount "20" @default.
• W157044733 countsByYear W1570447332012 @default.
• W157044733 countsByYear W1570447332014 @default.
• W157044733 countsByYear W1570447332016 @default.
• W157044733 countsByYear W1570447332017 @default.
• W157044733 countsByYear W1570447332018 @default.
• W157044733 countsByYear W1570447332021 @default.
• W157044733 countsByYear W1570447332023 @default.
• W157044733 crossrefType "journal-article" @default.
• W157044733 hasAuthorship W157044733A5004282838 @default.
• W157044733 hasAuthorship W157044733A5009018617 @default.
• W157044733 hasAuthorship W157044733A5030350457 @default.
• W157044733 hasAuthorship W157044733A5042490200 @default.
• W157044733 hasAuthorship W157044733A5060600247 @default.
• W157044733 hasBestOaLocation W1570447331 @default.
• W157044733 hasConcept C10389963 @default.
• W157044733 hasConcept C104317684 @default.
• W157044733 hasConcept C153911025 @default.
• W157044733 hasConcept C159654299 @default.
• W157044733 hasConcept C185592680 @default.
• W157044733 hasConcept C203014093 @default.
• W157044733 hasConcept C204628709 @default.
• W157044733 hasConcept C2776062316 @default.
• W157044733 hasConcept C2778910516 @default.
• W157044733 hasConcept C2780420688 @default.
• W157044733 hasConcept C2781065037 @default.
• W157044733 hasConcept C2992956773 @default.
• W157044733 hasConcept C523546767 @default.
• W157044733 hasConcept C542903549 @default.
• W157044733 hasConcept C54355233 @default.
• W157044733 hasConcept C547475151 @default.
• W157044733 hasConcept C55493867 @default.
• W157044733 hasConcept C86803240 @default.
• W157044733 hasConcept C89423630 @default.
• W157044733 hasConceptScore W157044733C10389963 @default.
• W157044733 hasConceptScore W157044733C104317684 @default.
• W157044733 hasConceptScore W157044733C153911025 @default.
• W157044733 hasConceptScore W157044733C159654299 @default.
• W157044733 hasConceptScore W157044733C185592680 @default.
• W157044733 hasConceptScore W157044733C203014093 @default.
• W157044733 hasConceptScore W157044733C204628709 @default.
• W157044733 hasConceptScore W157044733C2776062316 @default.
• W157044733 hasConceptScore W157044733C2778910516 @default.
• W157044733 hasConceptScore W157044733C2780420688 @default.
• W157044733 hasConceptScore W157044733C2781065037 @default.
• W157044733 hasConceptScore W157044733C2992956773 @default.
• W157044733 hasConceptScore W157044733C523546767 @default.
• W157044733 hasConceptScore W157044733C542903549 @default.
• W157044733 hasConceptScore W157044733C54355233 @default.
• W157044733 hasConceptScore W157044733C547475151 @default.
• W157044733 hasConceptScore W157044733C55493867 @default.
• W157044733 hasConceptScore W157044733C86803240 @default.
• W157044733 hasConceptScore W157044733C89423630 @default.
• W157044733 hasIssue "11" @default.
• W157044733 hasLocation W1570447331 @default.
• W157044733 hasLocation W1570447332 @default.
• W157044733 hasOpenAccess W157044733 @default.
• W157044733 hasPrimaryLocation W1570447331 @default.

• next