FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570480791> ?p ?o ?g. }

• W1570480791 endingPage "9594" @default.
• W1570480791 startingPage "9589" @default.
• W1570480791 abstract "Bovine lung cGMP-binding cGMP-specific phosphodiesterase (cG-BPDE) is a potent and relatively specific substrate for cGMP-dependent protein kinase (cGK) as compared to cAMP-dependent protein kinase (cAK) (Thomas, M. K., Francis, S. H., and Corbin, J. D. (1990) J. Biol. Chem. 265, 14971-14978). A synthetic peptide, RKISASEFDRPLR (BPDEtide), was synthesized corresponding to the sequence surrounding the phosphorylation site in cG-BPDE. BPDEtide retained the cGK/cAK kinase specificity demonstrated by native cG-BPDE: the apparent Km of BPDEtide for cGK was 5-fold lower than that for cAK (Km = 68 and 320 microM, respectively). Vmax values were 11 mumol/min/mg for cGK and 3.2 mumol/min/mg for cAK. The peptide was not phosphorylated to a measurable extent by protein kinase C or by calcium/calmodulin-dependent protein kinase II. Thus, the primary amino acid sequence of the peptide substrate was sufficient to confer kinase specificity. Studies in crude tissue extracts indicated that BPDEtide was the most selective peptide substrate documented for measuring cGK activity. Peptide analogs of BPDEtide were synthesized to determine the contribution of specific residues to cGK or cAK substrate specificity. Substitution of a Lys for the amino-terminal Arg did not reduce cGK/cAK specificity; neither did the exchange of an Ala for the non-phosphorylated Ser nor the removal of the 3 carboxyl-terminal residues. A truncated BPDEtide (RKISASE) served equally well as substrate (Km approximately 90 microM) for both kinases. However, restoration of the Phe, to yield RKISASEF, reproduced the original cGK/cAK specificity for BPDEtide (Km = 120 and 480 microM, respectively), primarily by decreasing the affinity of cAK. Addition of a carboxyl-terminal Phe to the peptide RKRSRAE (derived from the sequence of the cGK phosphorylation site in histone H2B) or to the peptide LRRASLG (derived from the sequence of the cAK phosphorylation site in pyruvate kinase) also improved the cGK/cAK specificity by decreasing the affinity of cAK. These data suggested that the Phe in each substrate tested is a negative determinant for cAK." @default.
• W1570480791 created "2016-06-24" @default.
• W1570480791 creator A5008985626 @default.
• W1570480791 creator A5012382947 @default.
• W1570480791 creator A5013110210 @default.
• W1570480791 creator A5032074339 @default.
• W1570480791 creator A5034675601 @default.
• W1570480791 creator A5036851298 @default.
• W1570480791 creator A5045331047 @default.
• W1570480791 date "1992-05-01" @default.
• W1570480791 modified "2023-09-27" @default.
• W1570480791 title "A phenylalanine in peptide substrates provides for selectivity between cGMP- and cAMP-dependent protein kinases." @default.
• W1570480791 cites W110557356 @default.
• W1570480791 cites W1490238718 @default.
• W1570480791 cites W1495691424 @default.
• W1570480791 cites W1504478980 @default.
• W1570480791 cites W1523809838 @default.
• W1570480791 cites W1550626564 @default.
• W1570480791 cites W1563765973 @default.
• W1570480791 cites W1566665463 @default.
• W1570480791 cites W1569489894 @default.
• W1570480791 cites W1570624812 @default.
• W1570480791 cites W1598689840 @default.
• W1570480791 cites W1607359602 @default.
• W1570480791 cites W1850214287 @default.
• W1570480791 cites W1978947297 @default.
• W1570480791 cites W1979111516 @default.
• W1570480791 cites W1991558363 @default.
• W1570480791 cites W2031910230 @default.
• W1570480791 cites W2057826234 @default.
• W1570480791 cites W2301599923 @default.
• W1570480791 doi "https://doi.org/10.1016/s0021-9258(19)50131-4" @default.
• W1570480791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1315760" @default.
• W1570480791 hasPublicationYear "1992" @default.
• W1570480791 type Work @default.
• W1570480791 sameAs 1570480791 @default.
• W1570480791 citedByCount "74" @default.
• W1570480791 countsByYear W15704807912012 @default.
• W1570480791 countsByYear W15704807912014 @default.
• W1570480791 countsByYear W15704807912017 @default.
• W1570480791 crossrefType "journal-article" @default.
• W1570480791 hasAuthorship W1570480791A5008985626 @default.
• W1570480791 hasAuthorship W1570480791A5012382947 @default.
• W1570480791 hasAuthorship W1570480791A5013110210 @default.
• W1570480791 hasAuthorship W1570480791A5032074339 @default.
• W1570480791 hasAuthorship W1570480791A5034675601 @default.
• W1570480791 hasAuthorship W1570480791A5036851298 @default.
• W1570480791 hasAuthorship W1570480791A5045331047 @default.
• W1570480791 hasBestOaLocation W15704807911 @default.
• W1570480791 hasConcept C118792377 @default.
• W1570480791 hasConcept C161790260 @default.
• W1570480791 hasConcept C184235292 @default.
• W1570480791 hasConcept C185592680 @default.
• W1570480791 hasConcept C2777431362 @default.
• W1570480791 hasConcept C2779281246 @default.
• W1570480791 hasConcept C515207424 @default.
• W1570480791 hasConcept C55493867 @default.
• W1570480791 hasConcept C97029542 @default.
• W1570480791 hasConceptScore W1570480791C118792377 @default.
• W1570480791 hasConceptScore W1570480791C161790260 @default.
• W1570480791 hasConceptScore W1570480791C184235292 @default.
• W1570480791 hasConceptScore W1570480791C185592680 @default.
• W1570480791 hasConceptScore W1570480791C2777431362 @default.
• W1570480791 hasConceptScore W1570480791C2779281246 @default.
• W1570480791 hasConceptScore W1570480791C515207424 @default.
• W1570480791 hasConceptScore W1570480791C55493867 @default.
• W1570480791 hasConceptScore W1570480791C97029542 @default.
• W1570480791 hasIssue "14" @default.
• W1570480791 hasLocation W15704807911 @default.
• W1570480791 hasOpenAccess W1570480791 @default.
• W1570480791 hasPrimaryLocation W15704807911 @default.
• W1570480791 hasRelatedWork W2021939235 @default.
• W1570480791 hasRelatedWork W2023938985 @default.
• W1570480791 hasRelatedWork W2160162460 @default.
• W1570480791 hasRelatedWork W2950422474 @default.
• W1570480791 hasRelatedWork W2974871632 @default.
• W1570480791 hasRelatedWork W3005527239 @default.
• W1570480791 hasRelatedWork W3130872260 @default.
• W1570480791 hasRelatedWork W3200462162 @default.
• W1570480791 hasRelatedWork W4240339722 @default.
• W1570480791 hasRelatedWork W817301665 @default.
• W1570480791 hasVolume "267" @default.
• W1570480791 isParatext "false" @default.
• W1570480791 isRetracted "false" @default.
• W1570480791 magId "1570480791" @default.
• W1570480791 workType "article" @default.