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• W1570484815 startingPage "234" @default.
• W1570484815 abstract "RNA labeled with [methyl-3H]methionine and/or [32P]orthophosphate was isolated from the polyribosomes of herpes simplex virus (HSV) types 1-infected cells and separated into polyadenylylated [poly(A+)]and non-polyadenylylated [poly(A-)] fractions. Virus-specific RNA was obtained by hybridization in liquid to either excess HSV DNA or filters containing immobilized HSV DNA. Analysis in denaturing sucrose gradients indicated that HSV-specific poly(A+) RNA sedimented in a broad peak, with a modal S value of 20. The ratio of [3H]methyl to 32P decreased with increasing size of RNA, suggesting that each RNA chain contains a similar sumber of methyl groups. Further analysis indicated an average of one RNase-resistant structure of the type m7G(5')pppNmpNp or m7G(5')pppNmpNmpNp per 2,780 nucleotides. The following components were identified in the 5'-terminal oligonucleotides of polyribosome-associated HSV-specific poly(A+) and poly(A-) RNA: 7-methylguanosine, N6,2'-O-dimethyladenosine, and the 2'-O-methyl derivatives of guanosine, adenosine, uridine, and denosine, and the 2'-O-methyl derivatives of guanosine, adenosine, uridine, and cytidine. The most common 5'-terminal sequences were m7G(5')pppm6Am and m7G(5')pppGm. An additional modified nucleoside, N6-methyladenosine, was present in an internal position of HSV-specific RNA." @default.
• W1570484815 created "2016-06-24" @default.
• W1570484815 creator A5028896105 @default.
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• W1570484815 creator A5035799441 @default.
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• W1570484815 creator A5083842825 @default.
• W1570484815 date "1977-08-01" @default.
• W1570484815 modified "2023-10-10" @default.
• W1570484815 title "5'-Terminal and internal methylated nucleosides in herpes simplex virus type 1 mRNA" @default.
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• W1570484815 doi "https://doi.org/10.1128/jvi.23.2.234-239.1977" @default.
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