Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570559562> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1570559562 endingPage "4503" @default.
- W1570559562 startingPage "4499" @default.
- W1570559562 abstract "The nucleotide affinity label 5'-p-fluorosulfonylbenzoyl adenosine reacts at the active site of rabbit muscle pyruvate kinase, with irreversible inactivation occurring concomitant with incorporation of about 1 mol of reagent/mol of enzyme subunit (Annamalai, A. E., and Colman, R. F. (1981) J. Biol. Chem. 256, 10276-10283). Purified peptides have now been isolated from 70% inactivated enzyme containing 0.7 mol of reagent/mol of enzyme subunit. Rabbit muscle enzyme labeled with radioactive 5'-p-fluorosulfonylbenzoyl adenosine was digested with thermolysin. Nucleosidyl peptides were purified by chromatography on phenylboronate-agarose and reverse-phase high performance liquid chromatography. After amino acid and N-terminal analysis, the peptides were identified by comparison with the primary sequences of chicken and cat muscle enzyme. About 75% of the reagent incorporated was distributed equally among three O-(4-carboxybenzenesulfonyl)tyrosine-containing peptides: Leu-Asp-CBS-Tyr-Lys-Asn, Val-CBS-Tyr, and Leu-Asp-Asn-Ala-CBS-Tyr. These tyrosines are located in a 28-residue segment of the 530-amino acid sequence. The remainder of the incorporation was found in two N epsilon-(4-carboxybenzenesulfonyl)lysine-containing peptides. Leu-CBS-Lys and Ala-CBS-Lys-Gly-Asp-Tyr-Pro. Modification in the presence of MnATP or MnADP resulted in a marked decrease in labeling of these peptides in proportion to the decreased inactivation. It is suggested that these modified residues are located in the region of the catalytically functional nucleotide binding site of pyruvate kinase." @default.
- W1570559562 created "2016-06-24" @default.
- W1570559562 creator A5043779329 @default.
- W1570559562 creator A5066027352 @default.
- W1570559562 date "1986-04-01" @default.
- W1570559562 modified "2023-10-16" @default.
- W1570559562 title "Identification of tyrosine and lysine peptides labeled by 5'-p-fluorosulfonylbenzoyl adenosine in the active site of pyruvate kinase." @default.
- W1570559562 cites W107336605 @default.
- W1570559562 cites W1183586821 @default.
- W1570559562 cites W1500855389 @default.
- W1570559562 cites W1501247974 @default.
- W1570559562 cites W1518904592 @default.
- W1570559562 cites W1523838318 @default.
- W1570559562 cites W1524236325 @default.
- W1570559562 cites W1540674180 @default.
- W1570559562 cites W1564838675 @default.
- W1570559562 cites W1574702409 @default.
- W1570559562 cites W1579954276 @default.
- W1570559562 cites W1599442262 @default.
- W1570559562 cites W1607910945 @default.
- W1570559562 cites W1621315209 @default.
- W1570559562 cites W1662971183 @default.
- W1570559562 cites W168438985 @default.
- W1570559562 cites W1976210127 @default.
- W1570559562 cites W1976231664 @default.
- W1570559562 cites W2038454646 @default.
- W1570559562 cites W2048437453 @default.
- W1570559562 cites W2050075757 @default.
- W1570559562 cites W2062825381 @default.
- W1570559562 cites W2064272336 @default.
- W1570559562 cites W2069290483 @default.
- W1570559562 cites W2074082505 @default.
- W1570559562 cites W2180056482 @default.
- W1570559562 cites W2312440545 @default.
- W1570559562 cites W2477291048 @default.
- W1570559562 cites W4293247451 @default.
- W1570559562 cites W7832712 @default.
- W1570559562 doi "https://doi.org/10.1016/s0021-9258(17)38528-9" @default.
- W1570559562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3082867" @default.
- W1570559562 hasPublicationYear "1986" @default.
- W1570559562 type Work @default.
- W1570559562 sameAs 1570559562 @default.
- W1570559562 citedByCount "19" @default.
- W1570559562 countsByYear W15705595622012 @default.
- W1570559562 countsByYear W15705595622014 @default.
- W1570559562 countsByYear W15705595622015 @default.
- W1570559562 countsByYear W15705595622023 @default.
- W1570559562 crossrefType "journal-article" @default.
- W1570559562 hasAuthorship W1570559562A5043779329 @default.
- W1570559562 hasAuthorship W1570559562A5066027352 @default.
- W1570559562 hasBestOaLocation W15705595621 @default.
- W1570559562 hasConcept C116834253 @default.
- W1570559562 hasConcept C181199279 @default.
- W1570559562 hasConcept C185592680 @default.
- W1570559562 hasConcept C20251656 @default.
- W1570559562 hasConcept C2776016237 @default.
- W1570559562 hasConcept C2776165026 @default.
- W1570559562 hasConcept C2776991684 @default.
- W1570559562 hasConcept C2780854706 @default.
- W1570559562 hasConcept C515207424 @default.
- W1570559562 hasConcept C55493867 @default.
- W1570559562 hasConcept C59822182 @default.
- W1570559562 hasConcept C86803240 @default.
- W1570559562 hasConceptScore W1570559562C116834253 @default.
- W1570559562 hasConceptScore W1570559562C181199279 @default.
- W1570559562 hasConceptScore W1570559562C185592680 @default.
- W1570559562 hasConceptScore W1570559562C20251656 @default.
- W1570559562 hasConceptScore W1570559562C2776016237 @default.
- W1570559562 hasConceptScore W1570559562C2776165026 @default.
- W1570559562 hasConceptScore W1570559562C2776991684 @default.
- W1570559562 hasConceptScore W1570559562C2780854706 @default.
- W1570559562 hasConceptScore W1570559562C515207424 @default.
- W1570559562 hasConceptScore W1570559562C55493867 @default.
- W1570559562 hasConceptScore W1570559562C59822182 @default.
- W1570559562 hasConceptScore W1570559562C86803240 @default.
- W1570559562 hasIssue "10" @default.
- W1570559562 hasLocation W15705595621 @default.
- W1570559562 hasOpenAccess W1570559562 @default.
- W1570559562 hasPrimaryLocation W15705595621 @default.
- W1570559562 hasRelatedWork W1518813207 @default.
- W1570559562 hasRelatedWork W1946101854 @default.
- W1570559562 hasRelatedWork W1968047324 @default.
- W1570559562 hasRelatedWork W1997122699 @default.
- W1570559562 hasRelatedWork W2006467666 @default.
- W1570559562 hasRelatedWork W2022674876 @default.
- W1570559562 hasRelatedWork W2028685109 @default.
- W1570559562 hasRelatedWork W2069291669 @default.
- W1570559562 hasRelatedWork W2401134112 @default.
- W1570559562 hasRelatedWork W2412924645 @default.
- W1570559562 hasVolume "261" @default.
- W1570559562 isParatext "false" @default.
- W1570559562 isRetracted "false" @default.
- W1570559562 magId "1570559562" @default.
- W1570559562 workType "article" @default.