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- W1570614779 abstract "A polymerase chain reaction (PCR) procedure is being developed to detect Escherichia coli O157:H7 and associated Shiga-like toxins (STX1 and STX2) directly from pork, bypassing the time-consuming microbial culture steps for identification. A triplex PCR procedure was adapted and conditions experimentally determined to efficiently amplify three target genes: uidA with unique sequence in E. coli O157:H7, and stx1 and stx2. A meat sample preparation technique developed in previous research was modified to allow detection of 0.2-2.0 colony forming units per gram (CFU./g.) of meat. Time required to complete the procedure after overnight incubation of samples is seven hours." @default.
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- W1570614779 date "1997-01-01" @default.
- W1570614779 modified "2023-09-26" @default.
- W1570614779 title "Triplex PCR for Rapid Detection of Escherichia coli O157:H7 Directly from Ground Pork" @default.
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