FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570674920> ?p ?o ?g. }

• W1570674920 abstract "HomeCirculationVol. 102, No. 20Heparin Also Interacts With Selectins and Modulates the Cell Adhesion Process Free AccessLetterPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyRedditDiggEmail Jump toFree AccessLetterPDF/EPUBHeparin Also Interacts With Selectins and Modulates the Cell Adhesion Process Kiyoyuki Yanaka Noriyuki Kato Tadao Nose Kiyoyuki YanakaKiyoyuki Yanaka Search for more papers by this author Noriyuki KatoNoriyuki Kato Search for more papers by this author Tadao NoseTadao Nose Department of Neurosurgery, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan, [email protected] Search for more papers by this author Originally published14 Nov 2000https://doi.org/10.1161/01.CIR.102.20.e169Circulation. 2000;102:e169To the Editor:We read with great interest the article by Peter and coworkers1 in the October 5, 1999 issue of Circulation in which the authors demonstrated the binding of heparin to integrin Mac-1 on stimulated leukocytes. Recent investigations have revealed that heparin can modulate biological processes, such as binding to adhesion receptors on endothelial cells and leukocytes.2 Leukocyte adhesion is a complex molecular process, and multiple adhesion receptor systems mediate the recruitment of leukocytes from the blood. The initial trafficking of circulating leukocytes to sites of inflammation is mediated by the selectin family of adhesion receptors; this is followed by the engagement of additional cellular recognition receptors, including the immunoglobulin superfamily and integrins. Heparin interacts with adhesion molecules, including integrin Mac-1 (CD11b/CD18) and selectins.34Peter and colleagues speculated that the binding of heparin to Mac-1 and the consequent inhibition of Mac-1 ligand binding could directly modulate coagulation, inflammation, and cell proliferation. They failed to mention the role of other adhesion molecules, such as selectins, in this process. We would like to bring to the authors’ attention our study on the role of heparin in the cell adhesion process.5 This study revealed that heparin inhibits leukocyte adhesion by antagonizing the function of selectins. We evaluated the efficacy of sulfated polysaccharides (unfractionated heparin, low-molecular-weight heparin, heparan sulfate, chondroitin sulfate, and dextran sulfate) on leukocyte accumulation in the infarcted brain and found that the administration of these sulfated polysaccharides led to reduced leukocyte accumulation. The relative potency of leukocyte accumulation inhibition of the sulfated polysaccharides tested was as follows: dextran sulfate ≥ unfractionated heparin > low-molecular-weight heparin ≥ chondroitin sulfate ≥ heparan sulfate. Potency was correlated with the molecule’s degree of sulfation. We hypothesized that leukocyte recruitment was due to an interaction between leukocyte selectins and carbohydrate ligands, such as the sulfated polysaccharide side chains of proteoglycans on the endothelial cell surface. Therefore, in addition to integrin Mac-1, selectins also play an important role in the cell adhesion process, and this promiscuous binding of heparin may modulate inflammation and cell proliferation. References 1 Peter K, Schwarz M, Conradt C, et al. Heparin inhibits ligand binding to the leukocyte integrin Mac-1 (CD11b/CD18). Circulation.1999; 100:1533–1539.CrossrefMedlineGoogle Scholar2 Nelson RM, Cecconi O, Roberts WG, et al. Heparin oligosaccharides bind L- and P-selectin and inhibit acute inflammation. Blood.1993; 82:3253–3258.MedlineGoogle Scholar3 Bârzu T, Van Rijn JL, Petitou M, et al. Endothelial binding sites for heparin: specificity and role in heparin neutralization. Biochem J.1986; 238:847–854.CrossrefMedlineGoogle Scholar4 Diamond MS, Alon R, Parkos CA, et al. Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD18). J Cell Biol.1995; 130:1473–1482.CrossrefMedlineGoogle Scholar5 Yanaka K, Spellman SR, McCarthy JB, et al. Reduction of brain injury using heparin to inhibit leukocyte accumulation in a rat model of transient focal cerebral ischemia, I: protective mechanism. J Neurosurg.1996; 85:1102–1107.CrossrefMedlineGoogle ScholarcirculationahaCirculationCirculationCirculation0009-73221524-4539Lippincott Williams & WilkinsResponsePeter Karlheinz, MD, Schwarz Meike, MD, Nordt Thomas, MD, Bode Christoph, MD, Conradt Christian, PhD, Moser Martin, MD, and Kübler Wolfgang, MD14112000We thank Yanaka et alR1 for their comments on the effects of heparin in cell adhesion processes. Indeed, because of space restrictions, we did not discuss the multiple functions of heparin in depth in our article.R2 We agree that the inhibition of P- and L-selectin by heparin may participate in the modulation of cell adhesion by heparin.Only a few reports have addressed the inhibition of cell adhesion by heparin. Most of these reports demonstrate an overall inhibitory effect of heparin on leukocyte recruitment, without defining the nature of the inhibition. For example, Yanaka et alR1 demonstrated that heparins inhibit leukocyte accumulation, reduce infarct size, and improve outcome in cerebral ischemia in a rat model. At least 2 reports define the inhibition of selectins as a potential mechanism by which heparin modulates cell adhesion. In an original approach, Nelson et alR3 demonstrated that heparins directly inhibit L- and P-selectin; they further demonstrated reduced neutrophil influx in an in vivo model of acute inflammation. Koenig et alR4 proved that L- and P-selectin were inhibited by heparins. For unfractionated heparin, the IC (concentration that inhibits 50%) was ≈10-fold less than the recommended levels for anticoagulation in an in vitro binding assay that evaluated L- and P-selectin–mediated cell adhesion.Two major adhesion steps of the cascade of leukocyte adhesion, the initial rolling that is mediated by selectins and the buildup of a firm adhesion that is mediated by integrins such as Mac-1, can be inhibited by heparin. As shown by us for the inhibition of Mac-1,R2 the inhibition of L- and P-selectin is also achieved at heparin concentrations commonly used in the clinic. Thus, besides its anticoagulative properties, heparin may provide significant benefits by the blocking leukocyte adhesion and function in clinical settings such as cerebral and myocardial ischemia, restenosis after angioplasty, and thrombosis. Previous Back to top Next FiguresReferencesRelatedDetails November 14, 2000Vol 102, Issue 20Article InformationMetrics Download: 119 Copyright © 2000 by American Heart Associationhttps://doi.org/10.1161/01.CIR.102.20.e169 Originally publishedNovember 14, 2000 PDF download Advertisement" @default.
• W1570674920 created "2016-06-24" @default.
• W1570674920 creator A5023548683 @default.
• W1570674920 creator A5027989105 @default.
• W1570674920 creator A5080626582 @default.
• W1570674920 date "2000-11-14" @default.
• W1570674920 modified "2023-10-16" @default.
• W1570674920 title "Heparin Also Interacts With Selectins and Modulates the Cell Adhesion Process" @default.
• W1570674920 cites W1528569576 @default.
• W1570674920 cites W1966126000 @default.
• W1570674920 cites W1973192371 @default.
• W1570674920 cites W2119132855 @default.
• W1570674920 cites W2150968653 @default.
• W1570674920 cites W73960470 @default.
• W1570674920 doi "https://doi.org/10.1161/01.cir.102.20.e169" @default.
• W1570674920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11076839" @default.
• W1570674920 hasPublicationYear "2000" @default.
• W1570674920 type Work @default.
• W1570674920 sameAs 1570674920 @default.
• W1570674920 citedByCount "4" @default.
• W1570674920 countsByYear W15706749202016 @default.
• W1570674920 crossrefType "journal-article" @default.
• W1570674920 hasAuthorship W1570674920A5023548683 @default.
• W1570674920 hasAuthorship W1570674920A5027989105 @default.
• W1570674920 hasAuthorship W1570674920A5080626582 @default.
• W1570674920 hasBestOaLocation W15706749201 @default.
• W1570674920 hasConcept C126322002 @default.
• W1570674920 hasConcept C1491633281 @default.
• W1570674920 hasConcept C155619193 @default.
• W1570674920 hasConcept C16224149 @default.
• W1570674920 hasConcept C170493617 @default.
• W1570674920 hasConcept C185592680 @default.
• W1570674920 hasConcept C195687474 @default.
• W1570674920 hasConcept C203014093 @default.
• W1570674920 hasConcept C2777557582 @default.
• W1570674920 hasConcept C55493867 @default.
• W1570674920 hasConcept C71924100 @default.
• W1570674920 hasConcept C85789140 @default.
• W1570674920 hasConcept C86803240 @default.
• W1570674920 hasConcept C91682701 @default.
• W1570674920 hasConcept C95444343 @default.
• W1570674920 hasConceptScore W1570674920C126322002 @default.
• W1570674920 hasConceptScore W1570674920C1491633281 @default.
• W1570674920 hasConceptScore W1570674920C155619193 @default.
• W1570674920 hasConceptScore W1570674920C16224149 @default.
• W1570674920 hasConceptScore W1570674920C170493617 @default.
• W1570674920 hasConceptScore W1570674920C185592680 @default.
• W1570674920 hasConceptScore W1570674920C195687474 @default.
• W1570674920 hasConceptScore W1570674920C203014093 @default.
• W1570674920 hasConceptScore W1570674920C2777557582 @default.
• W1570674920 hasConceptScore W1570674920C55493867 @default.
• W1570674920 hasConceptScore W1570674920C71924100 @default.
• W1570674920 hasConceptScore W1570674920C85789140 @default.
• W1570674920 hasConceptScore W1570674920C86803240 @default.
• W1570674920 hasConceptScore W1570674920C91682701 @default.
• W1570674920 hasConceptScore W1570674920C95444343 @default.
• W1570674920 hasIssue "20" @default.
• W1570674920 hasLocation W15706749201 @default.
• W1570674920 hasLocation W15706749202 @default.
• W1570674920 hasOpenAccess W1570674920 @default.
• W1570674920 hasPrimaryLocation W15706749201 @default.
• W1570674920 hasRelatedWork W1887034724 @default.
• W1570674920 hasRelatedWork W1965334235 @default.
• W1570674920 hasRelatedWork W2039284154 @default.
• W1570674920 hasRelatedWork W2107396952 @default.
• W1570674920 hasRelatedWork W2127961356 @default.
• W1570674920 hasRelatedWork W2399847421 @default.
• W1570674920 hasRelatedWork W2405602360 @default.
• W1570674920 hasRelatedWork W2414437576 @default.
• W1570674920 hasRelatedWork W2187096882 @default.
• W1570674920 hasRelatedWork W2492568271 @default.
• W1570674920 hasVolume "102" @default.
• W1570674920 isParatext "false" @default.
• W1570674920 isRetracted "false" @default.
• W1570674920 magId "1570674920" @default.
• W1570674920 workType "article" @default.