FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570737581> ?p ?o ?g. }

• W1570737581 abstract "(R,R9)-4′-methoxy-1-naphtylfenoterol (MNF) inhibits proliferation of 1321N1 astrocytoma cell line in a β2-adrenergic receptor (β2AR)-dependent manner, while reducing rat C6 glioma growth in a xenograft mouse model. MNF interferes also with pro-oncogenic signaling induced by the orphan G-protein-linked receptor, GPR55, in human HepG2 hepatocarcinoma and PANC-1 pancreatic carcinoma cells. Here, we utilized a number of cellular and biochemical techniques to investigate the anti-tumorigenic potential of MNF in a panel of human melanoma cell lines. Cell migration, which was performed using scratch wound healing assay, demonstrated the ability of MNF to dose-dependently reduce the motility of EGFR-expressing UACC-647 and M93-047 cell lines in a biphasic (IC50 = 0.32 nM and 1.48 µM) and sigmoidal (IC50 = 7.14 nM) fashion, respectively. In contrast, EGFR-negative UACC-903 cells were unresponsive to MNF. The proliferation rate of UACC-647 cells was diminished by 38 ± 3.7% in the presence of MNF, with an IC50 of 136 nM. Pharmacological inhibition of β2AR with the selective antagonist ICI-118,551 (50 nM) did not confer protection against the actions of MNF on cell motility and proliferation. U-shaped dose-response curves, with a nadir at 1 nM, were observed when the levels of phosphorylated MEK and ERK were determined in MNF-treated UACC-647 cells. A similar dose-response relationship was observed with the β2AR agonists, epinephrine and (R,R9)-fenoterol, and cell pretreatment with 50 nM ICI-118,551 abolished MNF-evoked drop in phospho-ERK levels. However, MNF markedly increased inhibitory phosphorylation of eukaryotic elongation factor 2 (eEF2) on Thr56, which was accompanied by global defect in protein biosynthesis as revealed by pulse-labeling experiments with [35S]-methionine. We conclude that the anti-tumorigenic functions of MNF most likely reflect dual effects of MNF through interaction with different receptors in melanoma. This work was supported entirely by the Intramural Research Program of the National Institute on Aging/NIH and NIA/NIH contract N01-AG31009, and the Foundation for Polish Science (TEAM 2009-4/5 programme). Citation Format: Artur Wnorowski, Rajib K. Paul, Lucita Jimenez, Lawrence Toll, Abdelmohsen Kotb, Michel Bernier, Irving W. Wainer. (R,R9)-4′-methoxy-1-naphtylfenoterol inhibits pro-survival signaling, proliferation and motility of select human melanoma cell lines. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3680. doi:10.1158/1538-7445.AM2014-3680" @default.
• W1570737581 created "2016-06-24" @default.
• W1570737581 creator A5008724133 @default.
• W1570737581 creator A5016648687 @default.
• W1570737581 creator A5020208450 @default.
• W1570737581 creator A5020423575 @default.
• W1570737581 creator A5022836657 @default.
• W1570737581 creator A5042630311 @default.
• W1570737581 creator A5073267357 @default.
• W1570737581 date "2014-09-30" @default.
• W1570737581 modified "2023-09-27" @default.
• W1570737581 title "Abstract 3680: (R,R')-4′-methoxy-1-naphtylfenoterol inhibits pro-survival signaling, proliferation and motility of select human melanoma cell lines" @default.
• W1570737581 doi "https://doi.org/10.1158/1538-7445.am2014-3680" @default.
• W1570737581 hasPublicationYear "2014" @default.
• W1570737581 type Work @default.
• W1570737581 sameAs 1570737581 @default.
• W1570737581 citedByCount "0" @default.
• W1570737581 crossrefType "proceedings-article" @default.
• W1570737581 hasAuthorship W1570737581A5008724133 @default.
• W1570737581 hasAuthorship W1570737581A5016648687 @default.
• W1570737581 hasAuthorship W1570737581A5020208450 @default.
• W1570737581 hasAuthorship W1570737581A5020423575 @default.
• W1570737581 hasAuthorship W1570737581A5022836657 @default.
• W1570737581 hasAuthorship W1570737581A5042630311 @default.
• W1570737581 hasAuthorship W1570737581A5073267357 @default.
• W1570737581 hasConcept C1491633281 @default.
• W1570737581 hasConcept C170493617 @default.
• W1570737581 hasConcept C185592680 @default.
• W1570737581 hasConcept C202751555 @default.
• W1570737581 hasConcept C2777752497 @default.
• W1570737581 hasConcept C502942594 @default.
• W1570737581 hasConcept C54355233 @default.
• W1570737581 hasConcept C55493867 @default.
• W1570737581 hasConcept C57074206 @default.
• W1570737581 hasConcept C58207958 @default.
• W1570737581 hasConcept C62112901 @default.
• W1570737581 hasConcept C62478195 @default.
• W1570737581 hasConcept C81885089 @default.
• W1570737581 hasConcept C86803240 @default.
• W1570737581 hasConcept C95444343 @default.
• W1570737581 hasConcept C98274493 @default.
• W1570737581 hasConceptScore W1570737581C1491633281 @default.
• W1570737581 hasConceptScore W1570737581C170493617 @default.
• W1570737581 hasConceptScore W1570737581C185592680 @default.
• W1570737581 hasConceptScore W1570737581C202751555 @default.
• W1570737581 hasConceptScore W1570737581C2777752497 @default.
• W1570737581 hasConceptScore W1570737581C502942594 @default.
• W1570737581 hasConceptScore W1570737581C54355233 @default.
• W1570737581 hasConceptScore W1570737581C55493867 @default.
• W1570737581 hasConceptScore W1570737581C57074206 @default.
• W1570737581 hasConceptScore W1570737581C58207958 @default.
• W1570737581 hasConceptScore W1570737581C62112901 @default.
• W1570737581 hasConceptScore W1570737581C62478195 @default.
• W1570737581 hasConceptScore W1570737581C81885089 @default.
• W1570737581 hasConceptScore W1570737581C86803240 @default.
• W1570737581 hasConceptScore W1570737581C95444343 @default.
• W1570737581 hasConceptScore W1570737581C98274493 @default.
• W1570737581 hasLocation W15707375811 @default.
• W1570737581 hasOpenAccess W1570737581 @default.
• W1570737581 hasPrimaryLocation W15707375811 @default.
• W1570737581 hasRelatedWork W1568278168 @default.
• W1570737581 hasRelatedWork W2008728948 @default.
• W1570737581 hasRelatedWork W2044852599 @default.
• W1570737581 hasRelatedWork W2050992006 @default.
• W1570737581 hasRelatedWork W2075343808 @default.
• W1570737581 hasRelatedWork W2129860672 @default.
• W1570737581 hasRelatedWork W2152875808 @default.
• W1570737581 hasRelatedWork W2239880021 @default.
• W1570737581 hasRelatedWork W2283614160 @default.
• W1570737581 hasRelatedWork W2334478639 @default.
• W1570737581 hasRelatedWork W2362252515 @default.
• W1570737581 hasRelatedWork W2393861268 @default.
• W1570737581 hasRelatedWork W2407278159 @default.
• W1570737581 hasRelatedWork W2519306844 @default.
• W1570737581 hasRelatedWork W2791514762 @default.
• W1570737581 hasRelatedWork W2902646572 @default.
• W1570737581 hasRelatedWork W2951725417 @default.
• W1570737581 hasRelatedWork W3033767885 @default.
• W1570737581 hasRelatedWork W3093071097 @default.
• W1570737581 hasRelatedWork W2761337883 @default.
• W1570737581 isParatext "false" @default.
• W1570737581 isRetracted "false" @default.
• W1570737581 magId "1570737581" @default.
• W1570737581 workType "article" @default.