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- W1570767078 abstract "An investigation was made of the kinetics of nucleic acid and protein (dry mass) synthesis during interphase of mouse fibroblasts in vitro. Quantitative microspectrophotometric and microinterferometric methods were employed for the determination of the cellular DNA, RNA and mass content. The time course of DNA, RNA and mass synthesis during interphase was obtained by analyzing the distribution of the DNA, RNA and mass content of individual cells from large samples in fixed populations. The main source of error in this type of analysis lies in momentary variation, i.e. variation in the amount of DNA, RNA and mass among cells in the same physiological stage of the cell cycle. The influence of momentary variation on this method of analysis was examined in order to be able to make corrections for it. The accuracy of the results obtained was discussed in relation to sample size and momentary variation. The G1, S and G2 periods were found to be 9, 6 and 4 hr respectively. During the S period DNA was synthesized at a constant rate. Contrary to DNA, RNA and mass were synthesized throughout the whole interphase. The rate of synthesis of both RNA and mass was 2–2.5 times faster at the end of interphase than it was at the beginning. The increase in the rate of RNA synthesis seemed to occur predominantly during the second half of interphase at the same time as the doubling of the amount of DNA in the cell. The rate of mass synthesis, however, seemed to increase over the whole of the interphase period in proportion to the amount of RNA." @default.
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- W1570767078 date "1965-08-01" @default.
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- W1570767078 title "Quantitative cytochemical studies on interphase growth" @default.
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- W1570767078 doi "https://doi.org/10.1016/0014-4827(65)90003-0" @default.
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