FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570774473> ?p ?o ?g. }

• W1570774473 abstract "In this thesis the occurrence of Bacillus cereus in the milk production and processing environment was investigated. Isolates were identified biochemically and by DNA probes based on the variable regions of 16S rRNA. Further characterization was carried out using biochemical and molecular typing, in order to determine the major contamination sources of milk. Furthermore, properties in relation to carbohydrate utilization, growth at low temperatures and enterotoxin production were examined. B. cereus is important as food spoilage organism. In the present study the microorganism was isolated from food ingredients such as yeast, flour, cacoa, herbs and spices. B. cereus was also found in a wide variety of processed food products including bakery products, Chinese meals, pasta products, chocolate and meat products. In pasteurized milk and dairy products, B. cereus was frequently present and it is well-known that it can be responsible for spoilage when post-heat- treatment contamination is absent. To enumerate spores in a sample, the most common procedure is to carry out a heat- activation treatment of 10 min at 80°C, followed by plating on a (selective) agar medium. To prevent germination of spores during sample preparation the time between the preparation of the primary dilution and heat-activation step should be less than 10 min and the temperature during the analysis should be as low as possible (e.g. by keeping dilutions in melting ice). After isolation, presumptive B. cereus are confirmed by biochemical tests, however, this may lead to incorrect identification. Several isolates, involved in food poisoning incidents, were shown to be B. thuringiensis, by sequencing part of the 16S rRNA. These results suggest that use of B. thuringiensis as insecticide may lead to foodborne infection or intoxication. To improve the confirmation procedure, we developed a specific and sensitive method, using DNA probes based on variable regions of the 16S rRNA, to differentiate between B. cereus and B. thuringiensis. On farms, B. cereus is introduced into raw milk by contamination of the udder with faeces, soil and, in winter, used bedding. In the dairy processing plants, additional contamination takes place via the equipment. Biochemical and growth characterization and molecular typing of isolates confirmed this and also showed that selection of strains occurs in the milk production and processing chain. Cleaning and disinfection will not eliminate all B. cereus in milking installations or heat exchangers, particularly not those adhering to surfaces of the equipment. Although only a few cases of milkborne infection and intoxication by B. cereus have been reported, most isolates were able to produce enterotoxin as determined by immunoblotting, cytotoxicity tests and PCR. However, if pasteurized milk is stored at 7°C and consumed within the best before date, this will not cause any problems for healthy adults." @default.
• W1570774473 created "2016-06-24" @default.
• W1570774473 creator A5035968834 @default.
• W1570774473 date "1997-01-01" @default.
• W1570774473 modified "2023-09-24" @default.
• W1570774473 title "Isolation, identification and characterization of Bacillus cereus from the dairy environment" @default.
• W1570774473 hasPublicationYear "1997" @default.
• W1570774473 type Work @default.
• W1570774473 sameAs 1570774473 @default.
• W1570774473 citedByCount "6" @default.
• W1570774473 countsByYear W15707744732013 @default.
• W1570774473 countsByYear W15707744732014 @default.
• W1570774473 crossrefType "journal-article" @default.
• W1570774473 hasAuthorship W1570774473A5035968834 @default.
• W1570774473 hasConcept C109097599 @default.
• W1570774473 hasConcept C112570922 @default.
• W1570774473 hasConcept C147490202 @default.
• W1570774473 hasConcept C174618031 @default.
• W1570774473 hasConcept C185592680 @default.
• W1570774473 hasConcept C18903297 @default.
• W1570774473 hasConcept C2777118263 @default.
• W1570774473 hasConcept C2780373777 @default.
• W1570774473 hasConcept C31903555 @default.
• W1570774473 hasConcept C523546767 @default.
• W1570774473 hasConcept C54355233 @default.
• W1570774473 hasConcept C58684123 @default.
• W1570774473 hasConcept C86803240 @default.
• W1570774473 hasConcept C89423630 @default.
• W1570774473 hasConceptScore W1570774473C109097599 @default.
• W1570774473 hasConceptScore W1570774473C112570922 @default.
• W1570774473 hasConceptScore W1570774473C147490202 @default.
• W1570774473 hasConceptScore W1570774473C174618031 @default.
• W1570774473 hasConceptScore W1570774473C185592680 @default.
• W1570774473 hasConceptScore W1570774473C18903297 @default.
• W1570774473 hasConceptScore W1570774473C2777118263 @default.
• W1570774473 hasConceptScore W1570774473C2780373777 @default.
• W1570774473 hasConceptScore W1570774473C31903555 @default.
• W1570774473 hasConceptScore W1570774473C523546767 @default.
• W1570774473 hasConceptScore W1570774473C54355233 @default.
• W1570774473 hasConceptScore W1570774473C58684123 @default.
• W1570774473 hasConceptScore W1570774473C86803240 @default.
• W1570774473 hasConceptScore W1570774473C89423630 @default.
• W1570774473 hasLocation W15707744731 @default.
• W1570774473 hasOpenAccess W1570774473 @default.
• W1570774473 hasPrimaryLocation W15707744731 @default.
• W1570774473 hasRelatedWork W140120010 @default.
• W1570774473 hasRelatedWork W1485044783 @default.
• W1570774473 hasRelatedWork W1573386145 @default.
• W1570774473 hasRelatedWork W1664364454 @default.
• W1570774473 hasRelatedWork W2060293044 @default.
• W1570774473 hasRelatedWork W2074749121 @default.
• W1570774473 hasRelatedWork W2083724479 @default.
• W1570774473 hasRelatedWork W2088949291 @default.
• W1570774473 hasRelatedWork W2101078558 @default.
• W1570774473 hasRelatedWork W2157879778 @default.
• W1570774473 hasRelatedWork W2244929688 @default.
• W1570774473 hasRelatedWork W2381379654 @default.
• W1570774473 hasRelatedWork W2383687568 @default.
• W1570774473 hasRelatedWork W2995912137 @default.
• W1570774473 hasRelatedWork W3008861258 @default.
• W1570774473 hasRelatedWork W3027487587 @default.
• W1570774473 hasRelatedWork W3088670526 @default.
• W1570774473 hasRelatedWork W3185922440 @default.
• W1570774473 hasRelatedWork W3204134040 @default.
• W1570774473 hasRelatedWork W2182864289 @default.
• W1570774473 isParatext "false" @default.
• W1570774473 isRetracted "false" @default.
• W1570774473 magId "1570774473" @default.
• W1570774473 workType "article" @default.