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• W1570776780 abstract "Ornithobacterium rhinotracheale (ORT) is a emerging gram negative bacterial pathogen in poultry. To facilitate the development of novel infection intervention and prevention strategies, the pathogenesis of ORT infection was investigated. In vitro infection assays demonstrated that ORT adhered to epithelial cells in a dose-dependent fashion. Comparison of the adhesive properties of different strains indicated that strain 41 lacked the ability to infect epithelial cells. This strain gained an adhesive phenotype when it was grown in suspension rather than on a solid phase medium. Bacterial adherence was inhibited in the presence of purified lipopolysaccharide (LPS). ORT also adhered to and became ingested by chicken and murine macrophages. This interaction was not blocked by LPS. The macrophage response to ORT was investigated using the production of nitric oxide (NO) as a marker. ORT induced the production of NO by macrophages. This stimulation did not require but was enhanced by rChIFN-gamma. The NO response stimulated by ORT differed from that induced by the Gramnegative bacteria E. coli and S.Enteritidis. The ORT response was slower and was not inhibited by the lipid A neutralizing agent polymixin B. LPS derived from ORT did stimulate NO production. The stimulation of NO production by ORT was specific for chicken macrophages. Both bovine and chicken serum inhibited ORT infection of epithelial cells. The inhibition was caused via an effect of serum factors on the adhesive properties of the bacteria. Biotinylated isolated serum glycoproteins showed direct binding of multiple serum glycoproteins to the bacteria. Serum inhibition was not observed in the interaction of ORT with macrophages. Competitive binding assays, in which the bacteria were first incubated with LPS or serum and subsequently with biotinylated isolated serum glycoproteins showed that LPS and serum components inhibited cellular infection via different mechanisms. Haemagglutination assays indicated variation among ORT strains to agglutinate erythrocytes. Strain 41 was unable to adhere to epithelial cells but did cause haemagglutination when the bacteria were grown onto solid phase media. When the bacteria were grown in suspension, opposite binding characteristics were observed. The apparent transition between phenotypes with different cell-type specificities (erythrocytes versus epithelial cells) thus varied with media growth conditions. To enable the use of molecualr biology in studying ORT pathogenesis, a transformation system was developed that was based on a cryptic plasmid isolated from ORT. This plasmid was completely sequenced and characterized and contained up to 14 different open reading frames of which some appear to encode the proteins involved the transport of heavy metals across the membrane. Introduction of the origin of replication and several regulatory sequences of this plasmid (designated pOR1) into the vector pGEM7, yielded a shuttle vector that replicated as an independent unit in both E. coli and ORT. An antibiotic resistance gene cfxA had been introduced into the plasmid and the transfer of this plasmid to confirm that the plasmid can be used as a genetic tool to identify novel bacterial virulence determinants and candidate vaccine antigens." @default.
• W1570776780 created "2016-06-24" @default.
• W1570776780 creator A5010522845 @default.
• W1570776780 date "2004-04-01" @default.
• W1570776780 modified "2023-09-24" @default.
• W1570776780 title "Molecular interaction of Ornithobacterium rhinotracheale with eukaryotic cells" @default.
• W1570776780 cites W145315035 @default.
• W1570776780 cites W1882790940 @default.
• W1570776780 cites W1925464617 @default.
• W1570776780 cites W1929009802 @default.
• W1570776780 cites W1964445882 @default.
• W1570776780 cites W1964470756 @default.
• W1570776780 cites W1966081732 @default.
• W1570776780 cites W1968902673 @default.
• W1570776780 cites W1971956126 @default.
• W1570776780 cites W1975085160 @default.
• W1570776780 cites W1976610502 @default.
• W1570776780 cites W1976907957 @default.
• W1570776780 cites W1985488268 @default.
• W1570776780 cites W1985782125 @default.
• W1570776780 cites W1986118886 @default.
• W1570776780 cites W1990802251 @default.
• W1570776780 cites W1999310173 @default.
• W1570776780 cites W2002313428 @default.
• W1570776780 cites W2004343006 @default.
• W1570776780 cites W2012536557 @default.
• W1570776780 cites W2012928154 @default.
• W1570776780 cites W2015218120 @default.
• W1570776780 cites W2023361821 @default.
• W1570776780 cites W2026104800 @default.
• W1570776780 cites W2027168837 @default.
• W1570776780 cites W2028371077 @default.
• W1570776780 cites W2030920336 @default.
• W1570776780 cites W2031104954 @default.
• W1570776780 cites W2034415638 @default.
• W1570776780 cites W2034602833 @default.
• W1570776780 cites W2035694562 @default.
• W1570776780 cites W2039154223 @default.
• W1570776780 cites W2043349190 @default.
• W1570776780 cites W2047358821 @default.
• W1570776780 cites W2052078119 @default.
• W1570776780 cites W2052545687 @default.
• W1570776780 cites W2052897651 @default.
• W1570776780 cites W2054404672 @default.
• W1570776780 cites W2056761032 @default.
• W1570776780 cites W2064590033 @default.
• W1570776780 cites W2064591169 @default.
• W1570776780 cites W2073132923 @default.
• W1570776780 cites W2079129309 @default.
• W1570776780 cites W2081850657 @default.
• W1570776780 cites W2086377425 @default.
• W1570776780 cites W2086427737 @default.
• W1570776780 cites W2088401855 @default.
• W1570776780 cites W2096453132 @default.
• W1570776780 cites W2100837269 @default.
• W1570776780 cites W2112084358 @default.
• W1570776780 cites W2113609437 @default.
• W1570776780 cites W2116485083 @default.
• W1570776780 cites W2121459138 @default.
• W1570776780 cites W2121769601 @default.
• W1570776780 cites W2125611262 @default.
• W1570776780 cites W2134040849 @default.
• W1570776780 cites W2138186830 @default.
• W1570776780 cites W2148814910 @default.
• W1570776780 cites W2149292020 @default.
• W1570776780 cites W2158421639 @default.
• W1570776780 cites W2165833539 @default.
• W1570776780 cites W2168197730 @default.
• W1570776780 cites W2169140191 @default.
• W1570776780 cites W2175532102 @default.
• W1570776780 cites W2177207199 @default.
• W1570776780 cites W2178134447 @default.
• W1570776780 cites W2180611214 @default.
• W1570776780 cites W2180732798 @default.
• W1570776780 cites W2186872642 @default.
• W1570776780 cites W2208953984 @default.
• W1570776780 cites W2312874240 @default.
• W1570776780 cites W2315767638 @default.
• W1570776780 cites W2317103657 @default.
• W1570776780 cites W2322498320 @default.
• W1570776780 cites W2322735872 @default.
• W1570776780 cites W2323600913 @default.
• W1570776780 cites W2328274601 @default.
• W1570776780 cites W2329011039 @default.
• W1570776780 cites W2329494117 @default.
• W1570776780 cites W2331279509 @default.
• W1570776780 cites W2335380428 @default.
• W1570776780 cites W2414925535 @default.
• W1570776780 cites W2419114769 @default.
• W1570776780 cites W287664784 @default.
• W1570776780 cites W3016827114 @default.
• W1570776780 cites W3196855927 @default.
• W1570776780 cites W33761236 @default.
• W1570776780 hasPublicationYear "2004" @default.
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