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- W1570790098 abstract "S -Nitrosoalbumin (SNALB) and S -[ 15 N]nitrosoalbumin (S[ 15 N]ALB) were prepared by various methods, purified and isolated by a novel selective extraction procedure using HiTrapBlue Sepharose affinity columns and characterized by various techniques including SDS-PAGE electrophoresis, UV–Vis spectroscopy and gas chromatography–mass spectrometry (GC–MS). S -Nitrosylation of albumin in freshly obtained human plasma by unlabeled and 15 N-labeled butylnitrite at neutral pH revealed the purest preparations. For GC–MS analysis, SNALB and S[ 15 N]ALB were treated with HgCl 2 to obtain nitrite and [ 15 N]nitrite, respectively, which were then analysed as their pentafluorobenzyl derivatives. S[ 15 N]ALB preparations were standardized by GC–MS using nitrite as internal standard. S[ 15 N]ALB was prepared and isolated at concentrations of 188±43 μ M (mean±SD, n =8) at a final yield of about 45%, an isotopic purity of 98%, and SDS-PAGE electrophoretic purity of 90%. 15 N-Labeled SNALB was used to study its metabolism in human blood. The half-life of S[ 15 N]ALB (25 μ M ) in human heparinized blood in vitro was determined by GC–MS as 5.5 h. The GC–MS method described here could be useful for the quantitative determination of SNALB in human plasma using S[ 15 N]ALB as an internal standard." @default.
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- W1570790098 date "1999-04-01" @default.
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- W1570790098 title "Measurement of S-nitrosoalbumin by gas chromatography–mass spectrometry" @default.
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- W1570790098 doi "https://doi.org/10.1016/s0378-4347(99)00011-0" @default.
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