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- W1570896222 abstract "The four essential methods for sperm antibody detection are considered and evaluated. The micro-sperm-agglutination and micro-sperm-immobilization tests have been improved and optimized. Quantitative estimation of sperm antibodies was made by an immunoelectrophoresis technique after separation of antibodies from the sera and genital tract secretions by affinity chromatography. Antibodies were typed by line-rocket immunoelectrophoresis. The pathologic errors of the modified tests were found to be small and the biologic errors were tolerable. Satisfactory results were achieved for sperm antibodies belonging to IgA and IgM, but not for other immunoglobulins.The four essential methods for sperm antibody detection -- the micro-sperm-agglutination test, the micro-sperm-immobilization test, affinity chromatography and rocket immunoelectrophoresis, and lone-rocket immunoelectrophoresis -- are considered and evaluated. 15 serum samples of female patients with sperm-agglutinating and sperm-immobilizing activity present were used to evaluate the reproducibility of the individual methods. The samples were selected from a group of 343 female patients with unknown causes of sterility. Samples from 5 different sperm donors had been tested in parallel on the same day and with the same donor on 4 different days to ensure quality control. Quantitative estimation of sperm antibodies was made by an immunoelectrophoresis technique after separation of antibodies from the sera and genital tract secretions by affinity chromatography. Antibodies were typed by line-rocket immunoelectrophoresis. The pathologic errors of the modified tests were found to be small and the biologic errors were tolerable. Satisfactory results were achieved for sperm antibodies belonging to IgA and IgM, but not for other immunoglobulins." @default.
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- W1570896222 date "1980-01-01" @default.
- W1570896222 modified "2023-10-17" @default.
- W1570896222 title "The occurrence of sperm antibodies in human reproduction" @default.
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- W1570896222 doi "https://doi.org/10.1016/0002-9378(80)90575-x" @default.
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