Matches in SemOpenAlex for { <https://semopenalex.org/work/W1570956348> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W1570956348 abstract "Abscission is an important process in the life cycle of a plant. It takes place in predetermined sites called Abscission Zones (AZs). In the previous study of our group, a potential abscission-related gene At1g64405 (G2) was identified of particular interest using a transcriptional analysis. The aim of this study was to characterize this gene in detail.Expression analysis of G2 was carried out by fusing its promoter with GUS or GFP. Reporter gene expression was detected specifically in floral organ AZ and cortical cells surrounding the sites of lateral roots emergence. Crosses were then carried out between G2:GUS plants and three important abscission mutants: ida, 35S:IDA and bop1/bop2 in order to further investigate the expression pattern of G2. The results, together with the bioinformatics analysis, indicate that G2 is specifically expressed in AZ and is an abscission-related gene, and reveal an inverse correlation between the expression of G2 and IDA. A gene manipulation strategy was then undertaken to generate the ectopically expressed and silenced lines of G2. Overexpression of G2 was achieved by fusing G2 to a 35S promoter whereas the null lines were obtained by an RNAi strategy. 35S:G2 plants displayed unusual root hair morphology while down-regulating G2 generated plants where pollen partially failed to develop. 35S:IDA mutants displayed phenotypes with earlier abscission, extended AZ, and the ectopic secretion of AGPs at the site of organ shedding. Ectopic expression of G2 in 35S:IDA plants partially suppressed these features. A bioinformatics analysis was performed to study G2 protein sequence in order to find out potential functional domains and four motifs were selected that may be important for protein function. The potential role of G2 will be discussed in detail in this thesis." @default.
- W1570956348 created "2016-06-24" @default.
- W1570956348 creator A5023363049 @default.
- W1570956348 date "2012-07-11" @default.
- W1570956348 modified "2023-09-24" @default.
- W1570956348 title "The characterization of a novel abscission-related gene in Arabidopsis thaliana" @default.
- W1570956348 hasPublicationYear "2012" @default.
- W1570956348 type Work @default.
- W1570956348 sameAs 1570956348 @default.
- W1570956348 citedByCount "0" @default.
- W1570956348 crossrefType "dissertation" @default.
- W1570956348 hasAuthorship W1570956348A5023363049 @default.
- W1570956348 hasConcept C104317684 @default.
- W1570956348 hasConcept C143065580 @default.
- W1570956348 hasConcept C150194340 @default.
- W1570956348 hasConcept C161733203 @default.
- W1570956348 hasConcept C166703698 @default.
- W1570956348 hasConcept C2775988993 @default.
- W1570956348 hasConcept C2779491563 @default.
- W1570956348 hasConcept C54355233 @default.
- W1570956348 hasConcept C59822182 @default.
- W1570956348 hasConcept C67705224 @default.
- W1570956348 hasConcept C86803240 @default.
- W1570956348 hasConcept C95444343 @default.
- W1570956348 hasConcept C97037327 @default.
- W1570956348 hasConcept C99705001 @default.
- W1570956348 hasConceptScore W1570956348C104317684 @default.
- W1570956348 hasConceptScore W1570956348C143065580 @default.
- W1570956348 hasConceptScore W1570956348C150194340 @default.
- W1570956348 hasConceptScore W1570956348C161733203 @default.
- W1570956348 hasConceptScore W1570956348C166703698 @default.
- W1570956348 hasConceptScore W1570956348C2775988993 @default.
- W1570956348 hasConceptScore W1570956348C2779491563 @default.
- W1570956348 hasConceptScore W1570956348C54355233 @default.
- W1570956348 hasConceptScore W1570956348C59822182 @default.
- W1570956348 hasConceptScore W1570956348C67705224 @default.
- W1570956348 hasConceptScore W1570956348C86803240 @default.
- W1570956348 hasConceptScore W1570956348C95444343 @default.
- W1570956348 hasConceptScore W1570956348C97037327 @default.
- W1570956348 hasConceptScore W1570956348C99705001 @default.
- W1570956348 hasLocation W15709563481 @default.
- W1570956348 hasOpenAccess W1570956348 @default.
- W1570956348 hasPrimaryLocation W15709563481 @default.
- W1570956348 hasRelatedWork W1587123730 @default.
- W1570956348 hasRelatedWork W1977773404 @default.
- W1570956348 hasRelatedWork W2016194472 @default.
- W1570956348 hasRelatedWork W2017998319 @default.
- W1570956348 hasRelatedWork W2030687403 @default.
- W1570956348 hasRelatedWork W2035212032 @default.
- W1570956348 hasRelatedWork W2054859075 @default.
- W1570956348 hasRelatedWork W205914002 @default.
- W1570956348 hasRelatedWork W2079241032 @default.
- W1570956348 hasRelatedWork W2092762710 @default.
- W1570956348 hasRelatedWork W2123507671 @default.
- W1570956348 hasRelatedWork W2142106846 @default.
- W1570956348 hasRelatedWork W2164207591 @default.
- W1570956348 hasRelatedWork W2164403189 @default.
- W1570956348 hasRelatedWork W2168910170 @default.
- W1570956348 hasRelatedWork W2270995240 @default.
- W1570956348 hasRelatedWork W2363763854 @default.
- W1570956348 hasRelatedWork W2614291425 @default.
- W1570956348 hasRelatedWork W3168181147 @default.
- W1570956348 hasRelatedWork W3199778952 @default.
- W1570956348 isParatext "false" @default.
- W1570956348 isRetracted "false" @default.
- W1570956348 magId "1570956348" @default.
- W1570956348 workType "dissertation" @default.