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- W1571402020 abstract "本研究发展了四种基于三羟基苯乙酮（THAP）的新型离子液体基质,即2′,3′,4′-THAP/二甲基苯胺（DMA）、2′,4′,6′-THAP/DMA、2′,3′,4′-THAP/吡啶（Py）及2′,4′,6′-THAP/Py,用于提高寡糖/糖肽在MALDI-TOF MS中的离子化效率.与传统的固体基质2,5-二羟基苯甲酸（DHB）和2′,4′,6′-三羟基苯乙酮（2′,4′,6′-THAP）相比,新型离子液体体系分析不同类型的寡糖链,均可获得更高的检测灵敏度.可使葡聚糖（dextran 1000）和环状寡聚糖β-环糊精在MALDI质谱中的信噪比提高10倍以上,RNase B的复杂寡糖链也实现了高灵敏度的检测.在糖肽分析中,2′,3′,4′-THAP/DMA离子液体高灵敏度地检测到辣根过氧化酶的7条糖肽,而2′,3′,4′-THAP作为基质时却无法检测到任何信号." @default.
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- W1571402020 date "2013-01-01" @default.
- W1571402020 modified "2023-10-01" @default.
- W1571402020 title "Novel Ionic Matrices for Enhanced Ionization of Oligosaccharides/Glycopeptides during MALDI-MS Analysis" @default.
- W1571402020 doi "https://doi.org/10.6023/a13030299" @default.
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