Matches in SemOpenAlex for { <https://semopenalex.org/work/W1571490147> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1571490147 endingPage "1397" @default.
- W1571490147 startingPage "1390" @default.
- W1571490147 abstract "Abstract Dihydrofolate reductase, isolated from baby hamster kidney cells by affinity chromatography, gave multiple bands on analytical gel electrophoresis and electrofocusing. The number of bands could be reduced to two by forming a complex with the enzyme inhibitor, methotrexate. The two basic enzyme forms, called Form I and Form II, were obtained in large quantities by preparative disc electrophoresis and analyzed for complex formation, aggregation, conformation, composition, catalytic activity, and interconversion. Each form produced stable and electrophoretically distinguishable complexes with substrate, substrate analogues, cofactor, and oxidized cofactor. The complex formation with the cofactors was accompanied by a change in the conformation. Form I and Form II differed in charge, conformation or size, specific activity, and pH optimum. They were not themselves cofactor complexes, nor was one of them a multimer or aggregate of the other. No interconversion could be shown. Analysis of the tryptic and chymotryptic digests of the two forms failed to show a difference in their amino acid sequence, but a small change in the sequence or certain physical or chemical modifications of the protein would not have been detected. We conclude that the multiple bands of dihydrofolate reductase are due to the presence of two closely related enzyme forms which independently produce stable and distinguishable complexes with the substrate, the cofactor, and the oxidized cofactor." @default.
- W1571490147 created "2016-06-24" @default.
- W1571490147 creator A5036187248 @default.
- W1571490147 creator A5079898951 @default.
- W1571490147 date "1974-03-01" @default.
- W1571490147 modified "2023-09-30" @default.
- W1571490147 title "Isolation and Characterization of the Multiple Forms of Dihydrofolate Reductase from Methotrexate-resistant Hamster Cells" @default.
- W1571490147 cites W1479742340 @default.
- W1571490147 cites W1492282552 @default.
- W1571490147 cites W1605791091 @default.
- W1571490147 cites W1766530870 @default.
- W1571490147 cites W1984696016 @default.
- W1571490147 cites W1986871364 @default.
- W1571490147 cites W2001079908 @default.
- W1571490147 cites W2001298057 @default.
- W1571490147 cites W2001855862 @default.
- W1571490147 cites W2006002532 @default.
- W1571490147 cites W2025320945 @default.
- W1571490147 cites W2026048218 @default.
- W1571490147 cites W2028426983 @default.
- W1571490147 cites W2032801740 @default.
- W1571490147 cites W2034226260 @default.
- W1571490147 cites W2038292406 @default.
- W1571490147 cites W2038345597 @default.
- W1571490147 cites W2049972861 @default.
- W1571490147 cites W2051757738 @default.
- W1571490147 cites W2057014506 @default.
- W1571490147 cites W2058428804 @default.
- W1571490147 cites W2058613232 @default.
- W1571490147 cites W2075882333 @default.
- W1571490147 cites W2087298783 @default.
- W1571490147 cites W2415250148 @default.
- W1571490147 doi "https://doi.org/10.1016/s0021-9258(19)42894-9" @default.
- W1571490147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4150389" @default.
- W1571490147 hasPublicationYear "1974" @default.
- W1571490147 type Work @default.
- W1571490147 sameAs 1571490147 @default.
- W1571490147 citedByCount "44" @default.
- W1571490147 crossrefType "journal-article" @default.
- W1571490147 hasAuthorship W1571490147A5036187248 @default.
- W1571490147 hasAuthorship W1571490147A5079898951 @default.
- W1571490147 hasBestOaLocation W15714901471 @default.
- W1571490147 hasConcept C153911025 @default.
- W1571490147 hasConcept C185592680 @default.
- W1571490147 hasConcept C203014093 @default.
- W1571490147 hasConcept C2775941552 @default.
- W1571490147 hasConcept C2778575167 @default.
- W1571490147 hasConcept C2781059491 @default.
- W1571490147 hasConcept C2781320022 @default.
- W1571490147 hasConcept C2909344506 @default.
- W1571490147 hasConcept C55493867 @default.
- W1571490147 hasConcept C86803240 @default.
- W1571490147 hasConcept C89423630 @default.
- W1571490147 hasConceptScore W1571490147C153911025 @default.
- W1571490147 hasConceptScore W1571490147C185592680 @default.
- W1571490147 hasConceptScore W1571490147C203014093 @default.
- W1571490147 hasConceptScore W1571490147C2775941552 @default.
- W1571490147 hasConceptScore W1571490147C2778575167 @default.
- W1571490147 hasConceptScore W1571490147C2781059491 @default.
- W1571490147 hasConceptScore W1571490147C2781320022 @default.
- W1571490147 hasConceptScore W1571490147C2909344506 @default.
- W1571490147 hasConceptScore W1571490147C55493867 @default.
- W1571490147 hasConceptScore W1571490147C86803240 @default.
- W1571490147 hasConceptScore W1571490147C89423630 @default.
- W1571490147 hasIssue "5" @default.
- W1571490147 hasLocation W15714901471 @default.
- W1571490147 hasOpenAccess W1571490147 @default.
- W1571490147 hasPrimaryLocation W15714901471 @default.
- W1571490147 hasRelatedWork W10219795 @default.
- W1571490147 hasRelatedWork W1823282561 @default.
- W1571490147 hasRelatedWork W1965076953 @default.
- W1571490147 hasRelatedWork W2033941503 @default.
- W1571490147 hasRelatedWork W2147932467 @default.
- W1571490147 hasRelatedWork W2327777985 @default.
- W1571490147 hasRelatedWork W2399434976 @default.
- W1571490147 hasRelatedWork W2410436151 @default.
- W1571490147 hasRelatedWork W2952663257 @default.
- W1571490147 hasRelatedWork W2079739363 @default.
- W1571490147 hasVolume "249" @default.
- W1571490147 isParatext "false" @default.
- W1571490147 isRetracted "false" @default.
- W1571490147 magId "1571490147" @default.
- W1571490147 workType "article" @default.