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• W157208099 endingPage "2002414" @default.
• W157208099 startingPage "2002414" @default.
• W157208099 abstract "Determining the amount of human DNA extracted from a crime scene sample is an important step in DNA profiling. The forensic community relies almost entirely upon a technique (slot blot) to quantitate human DNA that is imprecise, time consuming, and labor intensive. We have previously described a method for quantitation of human DNA based on PCR amplification of a repetitive Alu sequence that uses a fluorescence plate reader. This manuscript describes and validates a variation of this assay using real-time PCR and SYBR Green I for quantitation. The advantages of the real-time assay over the plate reader assay are: reduced hands-on time, lower assay cost, and a greater dynamic range. The main disadvantage is the cost of the real-time instrument. However, for those forensic laboratories with access to a real-time instrument, this Alu-based assay has a dynamic range of 16 ng to 1 pg, is sensitive, specific, fast, quantitative, and uses only 2 microL of sample." @default.
• W157208099 created "2016-06-24" @default.
• W157208099 creator A5038986782 @default.
• W157208099 creator A5065829077 @default.
• W157208099 date "2003-09-01" @default.
• W157208099 modified "2023-10-13" @default.
• W157208099 title "Development of an Alu-based, Real-Time PCR Method for Quantitation of Human DNA in Forensic Samples" @default.
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• W157208099 doi "https://doi.org/10.1520/jfs2002414" @default.
• W157208099 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14535658" @default.
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