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- W1572241925 abstract "In cells and cell-free extracts, the early steps in histone mRNA decay occur at the 3' terminus and appear to be catalyzed by a polysome-associated 3' to 5' exoribonuclease. We describe the purification of a polysomal 3' to 5' exoribonuclease that is magnesium-dependent, active at pH 7-8 in salt concentrations below 200 mM, and resistant to the inhibitor of the RNase A family of RNases. The purified enzyme is inactive with 3'-phosphorylated RNA substrates and with DNA but can degrade duplex RNA in the absence of added ATP. The enzyme migrates at approximately 37 kDa by native state gel filtration and at 33 kDa in a SDS-polyacrylamide gel. It degrades poly(A) but not a complex of poly(A) with poly(A) binding protein, and it accelerates histone mRNA decay in high salt-washed (enzyme-depleted) polysomes. Similarities between the purified exoribonuclease and the activity that degrades histone mRNA in vitro suggest that the enzyme might be a mammalian messenger ribonuclease." @default.
- W1572241925 created "2016-06-24" @default.
- W1572241925 creator A5020354916 @default.
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- W1572241925 date "1994-12-01" @default.
- W1572241925 modified "2023-10-18" @default.
- W1572241925 title "Purification of a human polyribosome-associated 3‘ to 5‘ exoribonuclease." @default.
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- W1572241925 doi "https://doi.org/10.1016/s0021-9258(18)31768-x" @default.
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