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- W1572650968 abstract "The presence of oestrogen receptor alpha (ER α )‐immunoreactivity (IR) in the cerebral cortex of adult rats has been investigated. Previous studies have reported a negligible presence of ER α or ER α mRNA in this region of the adult rat brain. We have used immunoprecipitation and immunohistochemistry, with various antibodies and fixatives, to detect this protein in the cingulate cortex. When the tissue was fixed using paraformaldehyde alone only faint ER α –IR was observed at this site. In contrast, following fixation either with acrolein (with or without paraformaldehyde) or with a mixture of paraformaldehyde and glutaraldehyde there was extensive ER α –IR throughout layers II to VI; this was absent when the antibodies were preincubated with the peptide fragment used in their production. The presence of ER α –IR in the nonfixed cingulate cortex of adult rats was confirmed by immunoprecipitation." @default.
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- W1572650968 date "1999-05-01" @default.
- W1572650968 modified "2023-10-18" @default.
- W1572650968 title "Evidence for Extensive Distribution of Oestrogen Receptor αImmunoreactivity in the Cerebral Cortex of Adult Rats" @default.
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- W1572650968 doi "https://doi.org/10.1046/j.1365-2826.1999.00346.x" @default.
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