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• W1573044600 abstract "ABSTRACT Aim: The kidney is a complex organ, requiring the contributions of multiple cell types to perform its various functions. Within this system the dendritic cell has been demonstrated to play a key role in maintaining the immunological balance of the kidney. In this methods paper we aim to identify the best method for isolating murine renal dendritic cells. Methods: The efficiency of isolating dendritic cells from enzymatically digested renal parenchyma by density centrifugation, MACS and FACS was compared. Results: Density centrifugation enriched dendritic cells by only approximately two fold. However, MACS and FACS resulted in a much higher purity (80% versus 95% respectively). Conclusions: Although FACS gave the highest purity, MACS is the optimal method for isolating dendritic cells given cost and time factors. Isolation of a homogeneous population of renal dendritic cells will enable the molecular and functional dissection of these cells in both homeostasis and disease models." @default.
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• W1573044600 date "2012-04-17" @default.
• W1573044600 modified "2023-10-16" @default.
• W1573044600 title "Isolation of functional dendritic cells from murine kidneys for immunological characterization" @default.
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• W1573044600 doi "https://doi.org/10.1111/j.1440-1797.2012.01581.x" @default.
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