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- W1573095611 abstract "5144 Progression of cancer results in the development of cancer cells with a metastatic potential from either mutation or adaptive changes phenotype at the primary site where the cancer arises. To enrich for these metastatic cells in an immunocompetent in vivo mouse model, we stably transfected mouse colon cancer MC-38 cells derived from C57/B16 mice with a luciferase reporter under control of the CMV promoter. Selection of highly invasive cells was accomplished in vitro through matrigel-coated polycarbonate filters in Transwells using a modified Boyden chamber invasion assay with serial enrichment of invasive cell populations through replicate selections. Bioluminescent imaging was used to monitor both in vitro invasion through the matrigel-coated Transwell filters and in vivo metastasis in syngeneic C57/B16 mice. MC-38 cells selected for invasion demonstrated a 15.2-fold increase in the number of invasive cells as compared with parental MC-38 cells. Equal number of the MC-38 parental cells or the invasion-enriched cells (MC-38inv) was injected into the tail vein, and C57/B16 mice were analyzed for in vivo bioluminescence weekly for three weeks (10 mice per group). At necropsy the number of pulmonary metastases per mouse in the group injected with MC-38inv cells averaged 14.2-fold greater and lung weight per mouse 2.6-fold greater than the group of mice injected with parental MC-38 cells. A cell line (now called MC-38Lu) was then established from a lung metastasis that developed after MC-38inv injection. These MC-38Lu cells were injected into tail vein to test formation of pulmonary metastasis in comparison with the MC-38 parental cells. After three weeks, the metastatic number resulting from MC-38Lu injection was too many to count. Lung weight per mouse for the MC-38Lu group increased 4.3-fold over parental MC-38 injected mice. Additionally, after the MC-38Lu cells were injected into the spleen, the mice developed numerous liver metastases that were too many to count. The liver weight per mouse was 3.6-fold greater than that of mice injected with MC-38 parental cells. Thus, this study establishes a reproducible metastastic model of colon cancer in syngeneic mice without the need for immunosuppression, providing an excellent opportunity to identify metastatic biomarkers and to study metastatic mechanisms in colon cancer. We are currently conducting genomic and proteomic studies of these metastatic cells." @default.
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- W1573095611 date "2006-04-15" @default.
- W1573095611 modified "2023-09-24" @default.
- W1573095611 title "Selection of a highly metastatic subpopulation of colon cancer cells in an in vivo immunocompetent mouse model." @default.
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