Matches in SemOpenAlex for { <https://semopenalex.org/work/W1573148085> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1573148085 endingPage "57" @default.
- W1573148085 startingPage "52" @default.
- W1573148085 abstract "Abstract A high performance liquid chromatographic method has been developed for the assay of arginine-specific ADP-ribosyl transferases. The assay utilizes L-arginine methyl ester (LAME) as the acceptor substrate. ADP-ribosylated-LAME is separated from the reaction mixture using a C-8 reversed-phase column. Before injection, the assay mixture is derivatized with an orthophthaldialdehyde/2-mercaptoethanol reagent. Fluorescence detection of the orthophthaldialdehyde-derivatized product provides excellent sensitivity and a limit of detection of less than 100 fmol. The kinetic mechanism of two arginine-specific ADP-ribosyltransferases, cholera toxin A subunit and an endogenous transferase from rabbit skeletal muscle, were both determined to be random sequential. The kinetic studies utilized 3-aminobenzamide and NG-monomethylarginine as competitive inhibitors for NAD and LAME, respectively. Cholera toxin was reported to have Km values of 5.6 and 39 mM for NAD and LAME, respectively. Km values of 0.56 and 1.2 mM were determined for NAD and LAME, respectively, using the transferase from rabbit skeletal muscle." @default.
- W1573148085 created "2016-06-24" @default.
- W1573148085 creator A5064344192 @default.
- W1573148085 creator A5065556359 @default.
- W1573148085 creator A5083634291 @default.
- W1573148085 date "1991-01-01" @default.
- W1573148085 modified "2023-09-28" @default.
- W1573148085 title "Determination of the kinetic mechanism of arginine-specific ADP-ribosyltransferases using a high performance liquid chromatographic assay." @default.
- W1573148085 cites W1483539527 @default.
- W1573148085 cites W1488591283 @default.
- W1573148085 cites W1500910904 @default.
- W1573148085 cites W1513226140 @default.
- W1573148085 cites W1514909941 @default.
- W1573148085 cites W1533877914 @default.
- W1573148085 cites W1592736409 @default.
- W1573148085 cites W1602192060 @default.
- W1573148085 cites W1935811184 @default.
- W1573148085 cites W1969582346 @default.
- W1573148085 cites W1972265920 @default.
- W1573148085 cites W1987328642 @default.
- W1573148085 cites W1987773676 @default.
- W1573148085 cites W1996896856 @default.
- W1573148085 cites W1998868558 @default.
- W1573148085 cites W205595150 @default.
- W1573148085 cites W2057243402 @default.
- W1573148085 cites W2077562485 @default.
- W1573148085 cites W2079491912 @default.
- W1573148085 cites W2280299329 @default.
- W1573148085 doi "https://doi.org/10.1016/s0021-9258(18)52400-5" @default.
- W1573148085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1898725" @default.
- W1573148085 hasPublicationYear "1991" @default.
- W1573148085 type Work @default.
- W1573148085 sameAs 1573148085 @default.
- W1573148085 citedByCount "14" @default.
- W1573148085 crossrefType "journal-article" @default.
- W1573148085 hasAuthorship W1573148085A5064344192 @default.
- W1573148085 hasAuthorship W1573148085A5065556359 @default.
- W1573148085 hasAuthorship W1573148085A5083634291 @default.
- W1573148085 hasBestOaLocation W15731480851 @default.
- W1573148085 hasConcept C111472728 @default.
- W1573148085 hasConcept C138885662 @default.
- W1573148085 hasConcept C185592680 @default.
- W1573148085 hasConcept C2777468819 @default.
- W1573148085 hasConcept C43617362 @default.
- W1573148085 hasConcept C515207424 @default.
- W1573148085 hasConcept C55493867 @default.
- W1573148085 hasConcept C89611455 @default.
- W1573148085 hasConceptScore W1573148085C111472728 @default.
- W1573148085 hasConceptScore W1573148085C138885662 @default.
- W1573148085 hasConceptScore W1573148085C185592680 @default.
- W1573148085 hasConceptScore W1573148085C2777468819 @default.
- W1573148085 hasConceptScore W1573148085C43617362 @default.
- W1573148085 hasConceptScore W1573148085C515207424 @default.
- W1573148085 hasConceptScore W1573148085C55493867 @default.
- W1573148085 hasConceptScore W1573148085C89611455 @default.
- W1573148085 hasIssue "1" @default.
- W1573148085 hasLocation W15731480851 @default.
- W1573148085 hasOpenAccess W1573148085 @default.
- W1573148085 hasPrimaryLocation W15731480851 @default.
- W1573148085 hasRelatedWork W1531601525 @default.
- W1573148085 hasRelatedWork W1990781990 @default.
- W1573148085 hasRelatedWork W2023079153 @default.
- W1573148085 hasRelatedWork W2024305086 @default.
- W1573148085 hasRelatedWork W2054443694 @default.
- W1573148085 hasRelatedWork W2607424097 @default.
- W1573148085 hasRelatedWork W2748952813 @default.
- W1573148085 hasRelatedWork W2899084033 @default.
- W1573148085 hasRelatedWork W2948807893 @default.
- W1573148085 hasRelatedWork W2778153218 @default.
- W1573148085 hasVolume "266" @default.
- W1573148085 isParatext "false" @default.
- W1573148085 isRetracted "false" @default.
- W1573148085 magId "1573148085" @default.
- W1573148085 workType "article" @default.