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• W1573368158 abstract "This chapter describes a detailed protocol for the expression and purification of full-length E2 or mutant E2 proteins lacking the classical N-terminal transactivation domain. The chapter describes experiments that allowed mapping of the segment of E2 responsible for the cooperative binding with TATA-binding protein (TBP). These mapping experiments identified a second activation domain of E2. To produce wild-type and N-terminal truncated forms of E2 in the yeast Saccharomyces cerevisiae, an inducible expression system is used. The E. coli/yeast shuttle vector pPD2 contains the PHO5 promoter, which is repressed in high phosphate medium and activated under conditions of limiting phosphate. The vector contains the yeast 2 μm element supporting replication in yeast and a leucine marker allowing selection for transformants in yeast strains auxotrophic for leucine. The chapter discusses the role of hinge region of E2 in transcriptional activation and in cooperative binding with TBP." @default.
• W1573368158 created "2016-06-24" @default.
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• W1573368158 date "1996-01-01" @default.
• W1573368158 modified "2023-10-16" @default.
• W1573368158 title "E2 proteins: Modulators of papillomavirus transcription and replication" @default.
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• W1573368158 doi "https://doi.org/10.1016/s0076-6879(96)74016-8" @default.
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