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- W1573729462 abstract "Peroxisomes are one of the main sites in the cell where oxygen free radicals are both generated and scavenged. The balance between these two processes is believed to be of great importance for proper functioning of cells and has been implicated in aging and carcinogenesis. To gain more insight in the functioning of the peroxisome, we developed a new method to measure the biophysical properties of these organelles in the living cell. To achieve this we made use of small fluorescently labelled polypeptides with a peroxisomal targeting signal of type I. Using such a probe, we determined the peroxisomal matrix is alkaline and that the peroxisomal membrane is impermeable to protons under physiological conditions. Oxidative stress deriving from peroxisomal b-oxidation is to be detoxified by a set of anti-oxidant enzymes. In this thesis I show that next to the well known enzymes catalase and MnSOD, the sterol carrier protein 2 (SCP2) plays a role in the protection against oxidative stress. We show that mice lacking this protein have higher basal levels of lipid-peroxidation in the liver and develop liver tumours. In a model I propose that this peroxisomal protein shields poly-unsaturated fatty acyl-CoA esters from free radicals that are present in the organellar matrix. The regulation of anti-oxidant activity in the cell has been a matter of study. We show that MnSOD is directly regulated by the daf-16 like Forkhead transcription factors. Forkhead transcription factors are negatively regulated by phosphatidyl-inositol-3-OH-kinase (PI-3-kinase) through phosphokinase B (PKB). Catalase and SCP2 are also increased upon activation of the Forkheads, but until now we do not know whether this reflects a direct regulation. A model is proposed for the regulation of anti-oxidant capacity of the cell in relation to metabolism." @default.
- W1573729462 created "2016-06-24" @default.
- W1573729462 creator A5027333160 @default.
- W1573729462 date "2001-09-27" @default.
- W1573729462 modified "2023-09-27" @default.
- W1573729462 title "The role of peroxisomes in oxidative stress" @default.
- W1573729462 cites W11411359 @default.
- W1573729462 cites W1275105234 @default.
- W1573729462 cites W147697280 @default.
- W1573729462 cites W1485176492 @default.
- W1573729462 cites W1492490882 @default.
- W1573729462 cites W1492622409 @default.
- W1573729462 cites W1494060254 @default.
- W1573729462 cites W1494453455 @default.
- W1573729462 cites W1496668015 @default.
- W1573729462 cites W1505147288 @default.
- W1573729462 cites W1521905573 @default.
- W1573729462 cites W1542263802 @default.
- W1573729462 cites W1553056390 @default.
- W1573729462 cites W1553075222 @default.
- W1573729462 cites W1556317573 @default.
- W1573729462 cites W1574033922 @default.
- W1573729462 cites W1585517331 @default.
- W1573729462 cites W1590755679 @default.
- W1573729462 cites W1600895371 @default.
- W1573729462 cites W1601351999 @default.
- W1573729462 cites W1607258414 @default.
- W1573729462 cites W1625966995 @default.
- W1573729462 cites W1631908837 @default.
- W1573729462 cites W1639582946 @default.
- W1573729462 cites W1647126819 @default.
- W1573729462 cites W171263212 @default.
- W1573729462 cites W17750692 @default.
- W1573729462 cites W1812352443 @default.
- W1573729462 cites W1813230101 @default.
- W1573729462 cites W1876742335 @default.
- W1573729462 cites W1877188956 @default.
- W1573729462 cites W1918864539 @default.
- W1573729462 cites W1964697500 @default.
- W1573729462 cites W1964727206 @default.
- W1573729462 cites W1964835198 @default.
- W1573729462 cites W1967503648 @default.
- W1573729462 cites W1967871887 @default.
- W1573729462 cites W1968346309 @default.
- W1573729462 cites W1970097948 @default.
- W1573729462 cites W1970788306 @default.
- W1573729462 cites W1972082708 @default.
- W1573729462 cites W1972711580 @default.
- W1573729462 cites W1972790402 @default.
- W1573729462 cites W1973596168 @default.
- W1573729462 cites W1973837499 @default.
- W1573729462 cites W1976050844 @default.
- W1573729462 cites W1976063015 @default.
- W1573729462 cites W1977055737 @default.
- W1573729462 cites W1977933239 @default.
- W1573729462 cites W1978265797 @default.
- W1573729462 cites W1982024631 @default.
- W1573729462 cites W1982547503 @default.
- W1573729462 cites W1983589581 @default.
- W1573729462 cites W1983763398 @default.
- W1573729462 cites W1984140370 @default.
- W1573729462 cites W1986492446 @default.
- W1573729462 cites W1986923259 @default.
- W1573729462 cites W1987049546 @default.
- W1573729462 cites W1987672004 @default.
- W1573729462 cites W1989282269 @default.
- W1573729462 cites W1989716587 @default.
- W1573729462 cites W1995933463 @default.
- W1573729462 cites W2000489985 @default.
- W1573729462 cites W2004078164 @default.
- W1573729462 cites W2005411425 @default.
- W1573729462 cites W2006120283 @default.
- W1573729462 cites W2007532475 @default.
- W1573729462 cites W2007604907 @default.
- W1573729462 cites W2009917506 @default.
- W1573729462 cites W2010687728 @default.
- W1573729462 cites W2010771420 @default.
- W1573729462 cites W2011534687 @default.
- W1573729462 cites W2011785467 @default.
- W1573729462 cites W2012326456 @default.
- W1573729462 cites W2012499725 @default.
- W1573729462 cites W2012573926 @default.
- W1573729462 cites W2014030740 @default.
- W1573729462 cites W2016418875 @default.
- W1573729462 cites W2016626029 @default.
- W1573729462 cites W2017061822 @default.
- W1573729462 cites W2017318500 @default.
- W1573729462 cites W2017517922 @default.
- W1573729462 cites W2018164553 @default.
- W1573729462 cites W2021286587 @default.
- W1573729462 cites W2022416092 @default.
- W1573729462 cites W2024220711 @default.
- W1573729462 cites W2026782080 @default.
- W1573729462 cites W2028266323 @default.
- W1573729462 cites W2028615614 @default.
- W1573729462 cites W2028891117 @default.
- W1573729462 cites W2032415729 @default.
- W1573729462 cites W2033722975 @default.
- W1573729462 cites W2034308170 @default.
- W1573729462 cites W2034365997 @default.