FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1573874857> ?p ?o ?g. }

• W1573874857 abstract "The vertebrate microbiome consists of the bacteria, fungi, archaea, protozoans, and viruses that inhabit the body at diverse locations including the skin, mouth, upper airways, urogenital tract, and digestive tract. These microorganisms are known to synthesize vitamins, interact with and tone the immune system, and dramatically affect human health. A long list of diseases has been associated with imbalances in commensal microbiome communities. The work presented in this dissertation aims to characterize the microeukaryotic and archaeal components of the gut microbiome through development of wet lab techniques and in silico methods, and apply them to the study of response to antibiotics. These methods provided a picture of the healthy fungal and archaeal communities in the gut, with high prevalence of the yeast Saccharomyces and the archaeon Methanobrevibactor, along with several other species. These new tools were then used to investigate the longitudinal changes that the microbiome undergoes when treated with heavy antibiotics. Using an antibiotic cocktail containing ampicillin, neomycin, vancomycin, and metronidazole in a mouse model, we found that bacterial communities were effectively suppressed and fungi grew out by one to two orders of magnitude. After we discontinued antibiotics, bacterial and fungal cell counts returned to baseline levels within one week, but community composition was still significantly altered. Eight weeks after cessation of antibiotics, fungal community composition was not significantly different from non-treated controls, but several mice continued to have elevated levels of yeasts that had grown out during antibiotic treatment. The bacterial community composition was still significantly different from non-treated controls. Ultimately, this work demonstrated potentially deleterious long term effects of antibiotic use, and emphasizes how strong cage effects can be in mouse studies. The research performed in this dissertation will aid researchers looking to study all three domains of life and take into account the effects of commonly used antibiotics in future microbiome studies. Degree Type Dissertation Degree Name Doctor of Philosophy (PhD) Graduate Group Genomics & Computational Biology First Advisor Frederic D. Bushman" @default.
• W1573874857 created "2016-06-24" @default.
• W1573874857 creator A5031545073 @default.
• W1573874857 date "2013-01-01" @default.
• W1573874857 modified "2023-09-27" @default.
• W1573874857 title "Dynamics of Microeukaryotes and Archaea in the Mammalian Gut Microbiome" @default.
• W1573874857 cites W131912594 @default.
• W1573874857 cites W145906557 @default.
• W1573874857 cites W1505804085 @default.
• W1573874857 cites W152085397 @default.
• W1573874857 cites W153937447 @default.
• W1573874857 cites W1573352190 @default.
• W1573874857 cites W1754015722 @default.
• W1573874857 cites W1796426005 @default.
• W1573874857 cites W1849607011 @default.
• W1573874857 cites W1864518864 @default.
• W1573874857 cites W1899785354 @default.
• W1573874857 cites W1913118204 @default.
• W1573874857 cites W1935827071 @default.
• W1573874857 cites W1946772113 @default.
• W1573874857 cites W194862172 @default.
• W1573874857 cites W1948637954 @default.
• W1573874857 cites W1963723331 @default.
• W1573874857 cites W1965399445 @default.
• W1573874857 cites W1965521901 @default.
• W1573874857 cites W1965846042 @default.
• W1573874857 cites W1965925525 @default.
• W1573874857 cites W1966369330 @default.
• W1573874857 cites W1967212083 @default.
• W1573874857 cites W1967821667 @default.
• W1573874857 cites W1968105193 @default.
• W1573874857 cites W1970678266 @default.
• W1573874857 cites W1970785533 @default.
• W1573874857 cites W1971449131 @default.
• W1573874857 cites W1972308191 @default.
• W1573874857 cites W1973311043 @default.
• W1573874857 cites W1975551477 @default.
• W1573874857 cites W1979449954 @default.
• W1573874857 cites W1979853218 @default.
• W1573874857 cites W1980411507 @default.
• W1573874857 cites W1980914602 @default.
• W1573874857 cites W1981436073 @default.
• W1573874857 cites W1981886126 @default.
• W1573874857 cites W1982367660 @default.
• W1573874857 cites W1982652385 @default.
• W1573874857 cites W1983950205 @default.
• W1573874857 cites W1984383838 @default.
• W1573874857 cites W1984420823 @default.
• W1573874857 cites W1984962510 @default.
• W1573874857 cites W1987635163 @default.
• W1573874857 cites W1988925586 @default.
• W1573874857 cites W1989889539 @default.
• W1573874857 cites W1990082546 @default.
• W1573874857 cites W1990127834 @default.
• W1573874857 cites W1990473825 @default.
• W1573874857 cites W1995781283 @default.
• W1573874857 cites W1998536115 @default.
• W1573874857 cites W1998926042 @default.
• W1573874857 cites W2000575260 @default.
• W1573874857 cites W2000589358 @default.
• W1573874857 cites W2001173856 @default.
• W1573874857 cites W2003364973 @default.
• W1573874857 cites W2003738804 @default.
• W1573874857 cites W2003836872 @default.
• W1573874857 cites W2005741884 @default.
• W1573874857 cites W2005982219 @default.
• W1573874857 cites W2006069224 @default.
• W1573874857 cites W2006465310 @default.
• W1573874857 cites W2006509711 @default.
• W1573874857 cites W2006696969 @default.
• W1573874857 cites W2007506470 @default.
• W1573874857 cites W2007890464 @default.
• W1573874857 cites W2009170813 @default.
• W1573874857 cites W2009880891 @default.
• W1573874857 cites W2010974592 @default.
• W1573874857 cites W2011996612 @default.
• W1573874857 cites W2014726754 @default.
• W1573874857 cites W2015550911 @default.
• W1573874857 cites W2015718756 @default.
• W1573874857 cites W2017973083 @default.
• W1573874857 cites W2018367988 @default.
• W1573874857 cites W2019746117 @default.
• W1573874857 cites W2020978031 @default.
• W1573874857 cites W2021064499 @default.
• W1573874857 cites W2022740153 @default.
• W1573874857 cites W2024248096 @default.
• W1573874857 cites W2029067777 @default.
• W1573874857 cites W2029310248 @default.
• W1573874857 cites W2030164702 @default.
• W1573874857 cites W2032097916 @default.
• W1573874857 cites W2032157122 @default.
• W1573874857 cites W2032230795 @default.
• W1573874857 cites W2033002674 @default.
• W1573874857 cites W2033647833 @default.
• W1573874857 cites W2035006556 @default.
• W1573874857 cites W2035779227 @default.
• W1573874857 cites W2036647435 @default.
• W1573874857 cites W2036743290 @default.
• W1573874857 cites W2037753451 @default.
• W1573874857 cites W2037878492 @default.

• next