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- W1574002301 abstract "Bioluminescence in the sea pansy, Renilla reniformis, a marine anthozoan coelenterate, is a complex process involving the participation of three proteins. These are: (1) the luciferin-binding protein, (2) the enzyme luciferase, and (3) the green-fluorescent protein (GFP). Luciferin-binding protein is a specific subtrate-binding protein which binds one molecule of coelenterate-type luciferin per molecule of protein and which then releases luciferin in the presence of Ca/sup 2 +/. Luciferase is the enzyme which catalyzes oxidation (by O/sub 2/) of coelenterate-type luciferin, leading to the production of CO/sub 2/ and enzyme-bound excited-state oxyluciferin. Oxyluciferin may then emit blue light by a direct de-excitation pathway or may transfer excitation energy to GFP. GFP is a noncatalytic accessory protein which accepts excitation energy from oxyluciferin, by radiationless energy transfer, and then emits green bioluminescence. In this paper the purification methods and physicochemical characteristics of GFP from R. reniformis are presented, and in the companion article luciferin-binding protein is described." @default.
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- W1574002301 date "1979-01-01" @default.
- W1574002301 modified "2023-09-27" @default.
- W1574002301 title "Energy transfer protein in coelenterate bioluminescence" @default.
- W1574002301 hasPublicationYear "1979" @default.
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