Matches in SemOpenAlex for { <https://semopenalex.org/work/W1574006175> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1574006175 endingPage "6852" @default.
- W1574006175 startingPage "6840" @default.
- W1574006175 abstract "Abstract The capacity of human peripheral blood (PB) dendritic cells (DC) and monocytes to facilitate T cell activation and the interaction molecules employed were compared. We have shown that precursors of DC constitute 2 to 3% of circulating PBMC, and can be isolated as CD33+CD14dim cells, whereas monocytes are CD33+CD14bright. Freshly obtained DC expressed similar densities of HLA-DR and the accessory molecules LFA-3, ICAM-1, and B7 as monocytes; after a 36-h incubation the expression of HLA-DR, ICAM-1, and B7 increased on both APC. Accessory cell function was examined in PB T cell cultures stimulated by suboptimal concentrations of immobilized mAb to CD3, and by stimulation of an allospecific T cell line. Freshly isolated monocytes and DC were comparable accessory cells in these assays, but their accessory function was increased by in vitro preincubation, although cultured DC and monocytes were comparably active. In contrast, DC were much more effective stimulators of freshly isolated allogeneic T cells than monocytes. DC were much more effective stimulators of freshly isolated allogeneic PB CD4+ naive and memory T cells than monocytes, whereas DC and monocytes were comparable accessory cells for memory and naive T cells stimulated with immobilized anti-CD3. The accessory molecules ICAM-1, LFA-3, and B7 were used comparably by DC and monocytes for accessory function in the presence of immobilized anti-CD3 and in the MLR, and none was unique for either APC population. These accessory molecules costimulated T cells in an additive fashion. Although immature blood DC and monocytes expressed minimal B7 and did not utilize it as an accessory molecule, B7 played an important role in the increased accessory function of differentiated APC. The results indicate that PB DC and monocytes function most efficiently after differentiation into mature cells that express increased amounts of MHC and other accessory molecules. Because DC and monocytes exhibit comparable accessory function in anti-CD3 T cell stimulation, differences in the expression of MHC molecules and/or their bound peptides are likely to explain the markedly enhanced capacity of DC to stimulate allogeneic PB T cells." @default.
- W1574006175 created "2016-06-24" @default.
- W1574006175 creator A5019607398 @default.
- W1574006175 creator A5043874873 @default.
- W1574006175 creator A5044211360 @default.
- W1574006175 date "1993-12-15" @default.
- W1574006175 modified "2023-10-13" @default.
- W1574006175 title "Comparative accessory cell function of human peripheral blood dendritic cells and monocytes." @default.
- W1574006175 doi "https://doi.org/10.4049/jimmunol.151.12.6840" @default.
- W1574006175 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8258694" @default.
- W1574006175 hasPublicationYear "1993" @default.
- W1574006175 type Work @default.
- W1574006175 sameAs 1574006175 @default.
- W1574006175 citedByCount "63" @default.
- W1574006175 countsByYear W15740061752013 @default.
- W1574006175 countsByYear W15740061752014 @default.
- W1574006175 countsByYear W15740061752015 @default.
- W1574006175 crossrefType "journal-article" @default.
- W1574006175 hasAuthorship W1574006175A5019607398 @default.
- W1574006175 hasAuthorship W1574006175A5043874873 @default.
- W1574006175 hasAuthorship W1574006175A5044211360 @default.
- W1574006175 hasBestOaLocation W15740061751 @default.
- W1574006175 hasConcept C114684123 @default.
- W1574006175 hasConcept C137061746 @default.
- W1574006175 hasConcept C147483822 @default.
- W1574006175 hasConcept C153911025 @default.
- W1574006175 hasConcept C154317977 @default.
- W1574006175 hasConcept C167672396 @default.
- W1574006175 hasConcept C202751555 @default.
- W1574006175 hasConcept C203014093 @default.
- W1574006175 hasConcept C2776090121 @default.
- W1574006175 hasConcept C2777367678 @default.
- W1574006175 hasConcept C2778170410 @default.
- W1574006175 hasConcept C2781184567 @default.
- W1574006175 hasConcept C2908647359 @default.
- W1574006175 hasConcept C39347974 @default.
- W1574006175 hasConcept C55493867 @default.
- W1574006175 hasConcept C67662055 @default.
- W1574006175 hasConcept C71924100 @default.
- W1574006175 hasConcept C86803240 @default.
- W1574006175 hasConcept C8891405 @default.
- W1574006175 hasConcept C95444343 @default.
- W1574006175 hasConcept C99454951 @default.
- W1574006175 hasConceptScore W1574006175C114684123 @default.
- W1574006175 hasConceptScore W1574006175C137061746 @default.
- W1574006175 hasConceptScore W1574006175C147483822 @default.
- W1574006175 hasConceptScore W1574006175C153911025 @default.
- W1574006175 hasConceptScore W1574006175C154317977 @default.
- W1574006175 hasConceptScore W1574006175C167672396 @default.
- W1574006175 hasConceptScore W1574006175C202751555 @default.
- W1574006175 hasConceptScore W1574006175C203014093 @default.
- W1574006175 hasConceptScore W1574006175C2776090121 @default.
- W1574006175 hasConceptScore W1574006175C2777367678 @default.
- W1574006175 hasConceptScore W1574006175C2778170410 @default.
- W1574006175 hasConceptScore W1574006175C2781184567 @default.
- W1574006175 hasConceptScore W1574006175C2908647359 @default.
- W1574006175 hasConceptScore W1574006175C39347974 @default.
- W1574006175 hasConceptScore W1574006175C55493867 @default.
- W1574006175 hasConceptScore W1574006175C67662055 @default.
- W1574006175 hasConceptScore W1574006175C71924100 @default.
- W1574006175 hasConceptScore W1574006175C86803240 @default.
- W1574006175 hasConceptScore W1574006175C8891405 @default.
- W1574006175 hasConceptScore W1574006175C95444343 @default.
- W1574006175 hasConceptScore W1574006175C99454951 @default.
- W1574006175 hasIssue "12" @default.
- W1574006175 hasLocation W15740061751 @default.
- W1574006175 hasLocation W15740061752 @default.
- W1574006175 hasOpenAccess W1574006175 @default.
- W1574006175 hasPrimaryLocation W15740061751 @default.
- W1574006175 hasRelatedWork W1505169546 @default.
- W1574006175 hasRelatedWork W1545583588 @default.
- W1574006175 hasRelatedWork W1574006175 @default.
- W1574006175 hasRelatedWork W1977136909 @default.
- W1574006175 hasRelatedWork W2020064966 @default.
- W1574006175 hasRelatedWork W2098907034 @default.
- W1574006175 hasRelatedWork W2107076397 @default.
- W1574006175 hasRelatedWork W2114826078 @default.
- W1574006175 hasRelatedWork W2317660163 @default.
- W1574006175 hasRelatedWork W2359198796 @default.
- W1574006175 hasVolume "151" @default.
- W1574006175 isParatext "false" @default.
- W1574006175 isRetracted "false" @default.
- W1574006175 magId "1574006175" @default.
- W1574006175 workType "article" @default.