FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1574129474> ?p ?o ?g. }

• W1574129474 endingPage "509" @default.
• W1574129474 startingPage "497" @default.
• W1574129474 abstract "We have investigated the mechanism by which double-stranded RNA (dsRNA) inhibits protein synthesis in cell-free extracts prepared from mouse L cells which had been pre-treated with purified interferon. Preincubation of such extracts for a determined period of time causes inactivation of tRNA species and hence an inhibition of protein synthesis with either Mengo RNA or poly(U,C) as mRNA. This inhibition can be overcome by addition of tRNA. We show that dsRNA inhibits the translation of Mengo RNA irrespective of the period of preincubation and that this inhibition is not overcome by tRNA. Translation of poly(U,C) is unaffected by dsRNA. Thus dsRNA does not act at the level of peptide chain elongation and does not cause inactivation of tRNA species, indicating that dsRNA inhibits protein synthesis in extracts of interferon-treated cells by a mechanism which is different from that responsible for the inhibition observed in the absence of dsRNA. The addition of dsRNA to extracts prepared from interferon-treated cells causes two modifications which are expected to inhibit protein synthesis. (a) An enhancement of the level of nuclease activity, causing Mengo [3H]RNA to be degraded more rapidly in interferon-treated cell extracts than control extracts, even when association of Mengo RNA with ribosomes is inhibited by the addition of pactamycin. (b) An impairment in the formation of methionyl-tRNA · 40-S-ribosomal-subunit complexes. The reduced binding of methionyl-tRNA to 40-S subunits can be overcome by the addition of a crude initiation factor preparation suggesting that dsRNA causes inactivation of an endogenous initiation factor(s). In the early stages of translation of Mengo RNA (10–15 min) dsRNA causes a reduction in the incorporation of [35S]methionine into the N-terminal position of polypeptides suggesting that initiation of protein synthesis is impaired, presumably as a result of the block of methionyl-tRNA · 40-S-subunit complex formation. At a later stage (30–45 min) Mengo RNA is almost completely destroyed under the normal conditions of protein synthesis." @default.
• W1574129474 created "2016-06-24" @default.
• W1574129474 creator A5005963577 @default.
• W1574129474 creator A5017461271 @default.
• W1574129474 creator A5029935997 @default.
• W1574129474 date "1978-05-01" @default.
• W1574129474 modified "2023-10-14" @default.
• W1574129474 title "Dual Action of Double-Stranded RNA in Inhibiting Protein Synthesis in Extracts of Interferon-Treated Mouse L Cells. Translation is Impaired at the Level of Initiation and by mRNA Degradation" @default.
• W1574129474 cites W1020566274 @default.
• W1574129474 cites W1180598583 @default.
• W1574129474 cites W1482953112 @default.
• W1574129474 cites W1507509336 @default.
• W1574129474 cites W1553730538 @default.
• W1574129474 cites W1579443142 @default.
• W1574129474 cites W1644684322 @default.
• W1574129474 cites W1839944039 @default.
• W1574129474 cites W1892457264 @default.
• W1574129474 cites W1937205275 @default.
• W1574129474 cites W1979000868 @default.
• W1574129474 cites W1981725382 @default.
• W1574129474 cites W1988186667 @default.
• W1574129474 cites W1988205258 @default.
• W1574129474 cites W1994630501 @default.
• W1574129474 cites W1994905155 @default.
• W1574129474 cites W1995014058 @default.
• W1574129474 cites W1995273385 @default.
• W1574129474 cites W1997142408 @default.
• W1574129474 cites W2003246132 @default.
• W1574129474 cites W2007124329 @default.
• W1574129474 cites W2013262809 @default.
• W1574129474 cites W2015718622 @default.
• W1574129474 cites W2021504472 @default.
• W1574129474 cites W2025496329 @default.
• W1574129474 cites W2026729363 @default.
• W1574129474 cites W2032807009 @default.
• W1574129474 cites W2053805303 @default.
• W1574129474 cites W2054666300 @default.
• W1574129474 cites W2061313150 @default.
• W1574129474 cites W2062417484 @default.
• W1574129474 cites W2066342343 @default.
• W1574129474 cites W2069514830 @default.
• W1574129474 cites W2072042076 @default.
• W1574129474 cites W2087297100 @default.
• W1574129474 cites W2099874422 @default.
• W1574129474 cites W2164102919 @default.
• W1574129474 doi "https://doi.org/10.1111/j.1432-1033.1978.tb12333.x" @default.
• W1574129474 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/207529" @default.
• W1574129474 hasPublicationYear "1978" @default.
• W1574129474 type Work @default.
• W1574129474 sameAs 1574129474 @default.
• W1574129474 citedByCount "49" @default.
• W1574129474 countsByYear W15741294742014 @default.
• W1574129474 crossrefType "journal-article" @default.
• W1574129474 hasAuthorship W1574129474A5005963577 @default.
• W1574129474 hasAuthorship W1574129474A5017461271 @default.
• W1574129474 hasAuthorship W1574129474A5029935997 @default.
• W1574129474 hasBestOaLocation W15741294741 @default.
• W1574129474 hasConcept C104317684 @default.
• W1574129474 hasConcept C105580179 @default.
• W1574129474 hasConcept C149364088 @default.
• W1574129474 hasConcept C153911025 @default.
• W1574129474 hasConcept C153957851 @default.
• W1574129474 hasConcept C159047783 @default.
• W1574129474 hasConcept C166703698 @default.
• W1574129474 hasConcept C181199279 @default.
• W1574129474 hasConcept C2191507 @default.
• W1574129474 hasConcept C2776178377 @default.
• W1574129474 hasConcept C2777271071 @default.
• W1574129474 hasConcept C3675279 @default.
• W1574129474 hasConcept C55493867 @default.
• W1574129474 hasConcept C67705224 @default.
• W1574129474 hasConcept C86803240 @default.
• W1574129474 hasConcept C88478588 @default.
• W1574129474 hasConceptScore W1574129474C104317684 @default.
• W1574129474 hasConceptScore W1574129474C105580179 @default.
• W1574129474 hasConceptScore W1574129474C149364088 @default.
• W1574129474 hasConceptScore W1574129474C153911025 @default.
• W1574129474 hasConceptScore W1574129474C153957851 @default.
• W1574129474 hasConceptScore W1574129474C159047783 @default.
• W1574129474 hasConceptScore W1574129474C166703698 @default.
• W1574129474 hasConceptScore W1574129474C181199279 @default.
• W1574129474 hasConceptScore W1574129474C2191507 @default.
• W1574129474 hasConceptScore W1574129474C2776178377 @default.
• W1574129474 hasConceptScore W1574129474C2777271071 @default.
• W1574129474 hasConceptScore W1574129474C3675279 @default.
• W1574129474 hasConceptScore W1574129474C55493867 @default.
• W1574129474 hasConceptScore W1574129474C67705224 @default.
• W1574129474 hasConceptScore W1574129474C86803240 @default.
• W1574129474 hasConceptScore W1574129474C88478588 @default.
• W1574129474 hasIssue "2" @default.
• W1574129474 hasLocation W15741294741 @default.
• W1574129474 hasLocation W15741294742 @default.
• W1574129474 hasOpenAccess W1574129474 @default.
• W1574129474 hasPrimaryLocation W15741294741 @default.
• W1574129474 hasRelatedWork W1987149792 @default.
• W1574129474 hasRelatedWork W1995014058 @default.
• W1574129474 hasRelatedWork W2058403594 @default.
• W1574129474 hasRelatedWork W2069600959 @default.

• next