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• W1574134877 abstract "Due to photobleaching and phototoxicity induced by high-intensity excitation light, the number of fluorescence images that can be obtained in live cells is always limited. This limitation becomes particularly prominent in multidimensional recordings when multiple Z-planes are captured at every time point. Here we present a simple technique, termed predictive-focus illumination (PFI), which helps to minimize cells’ exposure to light by decreasing the number of Z-planes that need to be captured in live-cell 3D time-lapse recordings. PFI utilizes computer tracking to predict positions of objects of interest (OOIs) and restricts image acquisition to small dynamic Z-regions centred on each OOI. Importantly, PFI does not require hardware modifications and it can be easily implemented on standard wide-field and spinning-disc confocal microscopes." @default.
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• W1574134877 date "2012-03-20" @default.
• W1574134877 modified "2023-10-16" @default.
• W1574134877 title "Predictive-focus illumination for reducing photodamage in live-cell microscopy" @default.
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• W1574134877 doi "https://doi.org/10.1111/j.1365-2818.2012.03605.x" @default.
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