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• W1574331073 abstract "ABSTRACT We provide novel data on vertebral ontogeny in the mouse, the mammalian model‐of‐choice for developmental studies. Most previous studies on ossification sequences in mice have focused on pooled elements of the spine (cervicals, thoracics, lumbars, sacrals, and caudals). Here, we contribute data on ossification sequences in the neural arches and centra to provide a comparative basis upon which to evaluate mammalian diversity of the axial skeleton. In attempt to explain the ossification pattern observed, we compared our observations with the phenotype of Cdx over‐expresser mice. We use high‐resolution X‐ray microtomography and clearing and staining techniques to quantify the precise sequential ossification pattern of the mouse spine. We show that micro‐CT scans perform better in all cases whereas clearing and staining exhibit sensitivity to the presence of semi‐opaque tissue. We observe that the centra of wild‐type mice always ossify after neural arches and that the ossification of the neural arches proceeds from two loci. The ossification of the centra appears more complex, especially in the neck where ossification is delayed and does not just follow the order of the vertebrae along the anterior–posterior axis. Our findings also suggest that Cdx genes' expression levels may be involved in the delayed ossification in the neck centra. J. Exp. Zool. (Mol. Dev. Evol.) 322B: 631–642, 2014 . © 2014 Wiley Periodicals, Inc." @default.
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• W1574331073 date "2014-08-11" @default.
• W1574331073 modified "2023-10-14" @default.
• W1574331073 title "Ossification sequence and genetic patterning in the mouse axial skeleton" @default.
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• W1574331073 doi "https://doi.org/10.1002/jez.b.22590" @default.
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