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- W1574378429 abstract "Three groups of proteins can be clearly discriminated in the total protein of L cell polysomes by selective labelling in the presence of low doses of actinomycin D and two-dimensional polyacrylamide/dodecylsulfate gel electrophoresis followed by autoradiography: (a) structural ribosomal proteins which are not labelled in the presence of actinomycin D and form stained non-radioactive spot in gels; (b) exchangeable ribosomal proteins which are labelled in the presence of actinomycin D and stained radioactive spots; (c) non-ribosomal proteins which are detectable only by autoradiography of gels. The large and small subunits of L cell ribosomes contain respectively 45 and 34 ribosomal proteins with molecular weights less than or equal to 50 000; seven of the large subunit proteins and nine of the small subunit proteins are exchangeable. Most of the non-ribosomal proteins migrate in the region of the related to the separation of the ribosomal proteins of mammalian cells and the possible significance of the presence of non-ribosomal proteins in polysomes are discussed." @default.
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- W1574378429 date "1979-01-01" @default.
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- W1574378429 title "The Polysomal Proteins of L Cells. Discrimination between the Structural Ribosomal Proteins, the Exchangeable Ribosomal Proteins and the Non-ribosomal Proteins by Two-Dimensional Dodecylsulfate Electrophoresis and Autoradiography" @default.
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- W1574378429 doi "https://doi.org/10.1111/j.1432-1033.1979.tb12790.x" @default.
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