FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1574396544> ?p ?o ?g. }

• W1574396544 abstract "Fluorescence recovery after photobleaching (FRAP) is a fluorescence microscope technique to measure molecular diffusion and transport. FRAP is a valuable technique in cell biological research and evolved conjointly with microscope and fluorescent probes advancements. Although developed in the 1970s, the discovery and further development of fluorescent proteins revolutionised FRAP. After the discovery of green fluorescence protein and its application as a noninvasive and genetically coded protein-tag, in vivo studies of protein dynamics and interactions became possible. FRAP is based on irreversibly bleaching a pool of fluorescent probes and monitoring the recovery in fluorescence due to movement of surrounding intact probes into the bleached spot. Although measurements are straightforward, quantitative FRAP requires careful experimental design, solid controls, data collection, and analysis. Over the past years, several FRAP-related techniques have been tailored to suit particular cell biological questions, including inverse FRAP, fluorescence loss in photobleaching, and fluorescence localisation after photobleaching.Key Concepts: Fluorescence recovery after photobleaching (FRAP) is a method to qualitatively and quantitatively study biomolecule dynamics in living cells.FRAP is based on irreversibly bleaching a pool of fluorescent probes with high intensity light and monitoring the recovery in fluorescence due to movement of surrounding intact probes into the bleached spot.FRAP experiments are often conducted on confocal microscopes. To derive quantitative results from such experiments, several parameters and controls need to be considered and utilised in the analysis.There are several FRAP-related methods that have been developed for specific applications and biological questions.FRAP is a versatile and popular method in modern biomedical research. Its application is broad and is increasingly applied in pharmacological, therapeutic and diagnostic areas.Keywords:FRAP;photobleaching;fluorescence;diffusion;protein dynamics;protein interaction;microscopy;confocal microscopy;fluorescent protein;GFP" @default.
• W1574396544 created "2016-06-24" @default.
• W1574396544 creator A5032441336 @default.
• W1574396544 creator A5049359623 @default.
• W1574396544 creator A5054780438 @default.
• W1574396544 date "2014-10-15" @default.
• W1574396544 modified "2023-09-24" @default.
• W1574396544 title "Fluorescence Recovery After Photobleaching (FRAP)" @default.
• W1574396544 cites W1496756449 @default.
• W1574396544 cites W1507696900 @default.
• W1574396544 cites W1524135328 @default.
• W1574396544 cites W1526551292 @default.
• W1574396544 cites W1536263551 @default.
• W1574396544 cites W1964409141 @default.
• W1574396544 cites W1966090424 @default.
• W1574396544 cites W1966975175 @default.
• W1574396544 cites W1968314092 @default.
• W1574396544 cites W1968392286 @default.
• W1574396544 cites W1985905499 @default.
• W1574396544 cites W1987187779 @default.
• W1574396544 cites W1988326309 @default.
• W1574396544 cites W1988401928 @default.
• W1574396544 cites W1988519425 @default.
• W1574396544 cites W1992004763 @default.
• W1574396544 cites W1993434836 @default.
• W1574396544 cites W1997452518 @default.
• W1574396544 cites W1998142826 @default.
• W1574396544 cites W1998195211 @default.
• W1574396544 cites W1998716065 @default.
• W1574396544 cites W1999860614 @default.
• W1574396544 cites W2007388359 @default.
• W1574396544 cites W2013984716 @default.
• W1574396544 cites W2015884353 @default.
• W1574396544 cites W2021823658 @default.
• W1574396544 cites W2025463860 @default.
• W1574396544 cites W2026896701 @default.
• W1574396544 cites W2027717396 @default.
• W1574396544 cites W2030239671 @default.
• W1574396544 cites W2032149639 @default.
• W1574396544 cites W2032300143 @default.
• W1574396544 cites W2032579724 @default.
• W1574396544 cites W2034844603 @default.
• W1574396544 cites W2037228253 @default.
• W1574396544 cites W2046291591 @default.
• W1574396544 cites W2051059195 @default.
• W1574396544 cites W2056104450 @default.
• W1574396544 cites W2056204193 @default.
• W1574396544 cites W2070005773 @default.
• W1574396544 cites W2077502289 @default.
• W1574396544 cites W2078922957 @default.
• W1574396544 cites W2080597633 @default.
• W1574396544 cites W2119737153 @default.
• W1574396544 cites W2127068865 @default.
• W1574396544 cites W2130547757 @default.
• W1574396544 cites W2140587321 @default.
• W1574396544 cites W2148303154 @default.
• W1574396544 cites W2149076845 @default.
• W1574396544 cites W2156457394 @default.
• W1574396544 cites W2168783089 @default.
• W1574396544 cites W2286479189 @default.
• W1574396544 cites W2405161561 @default.
• W1574396544 cites W2409867557 @default.
• W1574396544 cites W2411602000 @default.
• W1574396544 cites W2413919649 @default.
• W1574396544 cites W2429130570 @default.
• W1574396544 doi "https://doi.org/10.1002/9780470015902.a0003114" @default.
• W1574396544 hasPublicationYear "2014" @default.
• W1574396544 type Work @default.
• W1574396544 sameAs 1574396544 @default.
• W1574396544 citedByCount "8" @default.
• W1574396544 countsByYear W15743965442020 @default.
• W1574396544 countsByYear W15743965442021 @default.
• W1574396544 crossrefType "reference-entry" @default.
• W1574396544 hasAuthorship W1574396544A5032441336 @default.
• W1574396544 hasAuthorship W1574396544A5049359623 @default.
• W1574396544 hasAuthorship W1574396544A5054780438 @default.
• W1574396544 hasConcept C120665830 @default.
• W1574396544 hasConcept C121332964 @default.
• W1574396544 hasConcept C12554922 @default.
• W1574396544 hasConcept C136009344 @default.
• W1574396544 hasConcept C147080431 @default.
• W1574396544 hasConcept C169274487 @default.
• W1574396544 hasConcept C185592680 @default.
• W1574396544 hasConcept C26659971 @default.
• W1574396544 hasConcept C2779178360 @default.
• W1574396544 hasConcept C31332276 @default.
• W1574396544 hasConcept C49853544 @default.
• W1574396544 hasConcept C55493867 @default.
• W1574396544 hasConcept C57477423 @default.
• W1574396544 hasConcept C67649825 @default.
• W1574396544 hasConcept C86803240 @default.
• W1574396544 hasConcept C91881484 @default.
• W1574396544 hasConcept C95444343 @default.
• W1574396544 hasConceptScore W1574396544C120665830 @default.
• W1574396544 hasConceptScore W1574396544C121332964 @default.
• W1574396544 hasConceptScore W1574396544C12554922 @default.
• W1574396544 hasConceptScore W1574396544C136009344 @default.
• W1574396544 hasConceptScore W1574396544C147080431 @default.
• W1574396544 hasConceptScore W1574396544C169274487 @default.
• W1574396544 hasConceptScore W1574396544C185592680 @default.

• next