Matches in SemOpenAlex for { <https://semopenalex.org/work/W1574643837> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1574643837 endingPage "33" @default.
- W1574643837 startingPage "4925" @default.
- W1574643837 abstract "Abstract The metabolism of the suspected carcinogen acrylonitrile was studied using subcellular fractions isolated from rats and humans. Irreversible binding of radioactive label from [1-14C]-or [2,3-14C]acrylonitrile to protein and DNA was enhanced by reduced nicotinamide adenine dinucleotide phosphate in the presence of rat liver microsomes or a reconstituted cytochrome P-450 system. During the reduced nicotinamide adenine dinucleotide phosphate-dependent reaction, HCN was produced, and the heme of cytochrome P-450 was destroyed. Rat brain microsomes did not produce detectable levels of metabolites. Conclusive evidence of metabolically mediated binding of acrylonitrile to protein and DNA in human systems was not found. With rats, metabolism was induced by pretreatment of animals with either phenobarbital or 5,6-benzoflavone. Labeled 2-cyanoethylene oxide was found to bind irreversibly to calf thymus DNA and microsomal protein. The extent of binding was greater in the case of 2,3-14C-labeled than 1-14C-labeled material. The relative first-order rate of acrylonitrile binding to calf thymus DNA in rat liver microsomal systems was one to two orders of magnitude less than that for 1,1,2-trichloroethylene and three orders of magnitude less than that for vinyl chloride or vinyl bromide. Rat liver microsomes or a reconstituted cytochrome P-450 system catalyzed the mixed-function oxidation of acrylonitrile to 2-cyanoethylene oxide. 2-Cyanoethylene oxide has a half-life of about 2 hr in neutral buffer at 37°. This epoxide was found to serve as a substrate for microsomal epoxide hydrolase. HCN was released during hydrolysis of 2-cyanoethylene oxide or reaction of the epoxide with reduced glutathione; however, HCN release in either case was not stoichiometric with epoxide disappearance. 2-Cyanoethylene oxide reacted less rapidly with reduced glutathione than did acrylonitrile. Rat liver cytosol preparations contained glutathione S-transferase activity towards acrylonitrile and greater activity with 2-cyanoethylene oxide as a substrate. Cytosol preparations from rat brain and human liver had no detectable glutathione S-transferase activity towards acrylonitrile but did exhibit some activity towards 2-cyanoethylene oxide. These studies establish the basic pathways involved in the metabolism of acrylonitrile and should provide a basis for examination of the relevance of these individual steps and their roles in bioactivation and detoxication under more physiological situations." @default.
- W1574643837 created "2016-06-24" @default.
- W1574643837 creator A5012675234 @default.
- W1574643837 creator A5039078054 @default.
- W1574643837 creator A5052410999 @default.
- W1574643837 creator A5076783149 @default.
- W1574643837 date "1981-12-01" @default.
- W1574643837 modified "2023-09-23" @default.
- W1574643837 title "In vitro metabolism of acrylonitrile to 2-cyanoethylene oxide, reaction with glutathione, and irreversible binding to proteins and nucleic acids." @default.
- W1574643837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6272984" @default.
- W1574643837 hasPublicationYear "1981" @default.
- W1574643837 type Work @default.
- W1574643837 sameAs 1574643837 @default.
- W1574643837 citedByCount "40" @default.
- W1574643837 countsByYear W15746438372015 @default.
- W1574643837 countsByYear W15746438372021 @default.
- W1574643837 crossrefType "journal-article" @default.
- W1574643837 hasAuthorship W1574643837A5012675234 @default.
- W1574643837 hasAuthorship W1574643837A5039078054 @default.
- W1574643837 hasAuthorship W1574643837A5052410999 @default.
- W1574643837 hasAuthorship W1574643837A5076783149 @default.
- W1574643837 hasConcept C101819947 @default.
- W1574643837 hasConcept C114246631 @default.
- W1574643837 hasConcept C15920480 @default.
- W1574643837 hasConcept C178790620 @default.
- W1574643837 hasConcept C181199279 @default.
- W1574643837 hasConcept C185592680 @default.
- W1574643837 hasConcept C197957613 @default.
- W1574643837 hasConcept C24107716 @default.
- W1574643837 hasConcept C2778372188 @default.
- W1574643837 hasConcept C2780768313 @default.
- W1574643837 hasConcept C38485361 @default.
- W1574643837 hasConcept C521977710 @default.
- W1574643837 hasConcept C538909803 @default.
- W1574643837 hasConcept C55493867 @default.
- W1574643837 hasConcept C62231903 @default.
- W1574643837 hasConcept C87644729 @default.
- W1574643837 hasConceptScore W1574643837C101819947 @default.
- W1574643837 hasConceptScore W1574643837C114246631 @default.
- W1574643837 hasConceptScore W1574643837C15920480 @default.
- W1574643837 hasConceptScore W1574643837C178790620 @default.
- W1574643837 hasConceptScore W1574643837C181199279 @default.
- W1574643837 hasConceptScore W1574643837C185592680 @default.
- W1574643837 hasConceptScore W1574643837C197957613 @default.
- W1574643837 hasConceptScore W1574643837C24107716 @default.
- W1574643837 hasConceptScore W1574643837C2778372188 @default.
- W1574643837 hasConceptScore W1574643837C2780768313 @default.
- W1574643837 hasConceptScore W1574643837C38485361 @default.
- W1574643837 hasConceptScore W1574643837C521977710 @default.
- W1574643837 hasConceptScore W1574643837C538909803 @default.
- W1574643837 hasConceptScore W1574643837C55493867 @default.
- W1574643837 hasConceptScore W1574643837C62231903 @default.
- W1574643837 hasConceptScore W1574643837C87644729 @default.
- W1574643837 hasIssue "12 Pt 1" @default.
- W1574643837 hasLocation W15746438371 @default.
- W1574643837 hasOpenAccess W1574643837 @default.
- W1574643837 hasPrimaryLocation W15746438371 @default.
- W1574643837 hasRelatedWork W1644857730 @default.
- W1574643837 hasRelatedWork W177218465 @default.
- W1574643837 hasRelatedWork W1986225060 @default.
- W1574643837 hasRelatedWork W2037071640 @default.
- W1574643837 hasRelatedWork W2048844965 @default.
- W1574643837 hasRelatedWork W2089967366 @default.
- W1574643837 hasRelatedWork W2119477379 @default.
- W1574643837 hasRelatedWork W2123903073 @default.
- W1574643837 hasRelatedWork W2439855242 @default.
- W1574643837 hasRelatedWork W4230242462 @default.
- W1574643837 hasVolume "41" @default.
- W1574643837 isParatext "false" @default.
- W1574643837 isRetracted "false" @default.
- W1574643837 magId "1574643837" @default.
- W1574643837 workType "article" @default.